Abstract
Synthetic antiferromagnetic (SAF) nanoparticles are layer-structured particles with high single-particle magnetic moments. In order to covalently bind these nanoparticles to cells, they were coated with a silica shell followed by conjugation with streptavidin. The silica coating generates both SAF@SiO2 core-shell nanoparticles and silica core-free nanoparticles. Using a simple magnetic separation, silica nanoparticles were removed and SAF@SiO2 nanoparticles were purified. After streptavidin conjugation, these particles were used to stain lung cancer cells, making them highly magnetically responsive. The stained cells can rotate in response to an external magnetic field and can be captured when a blood sample containing these cells flows through the sifter.
Similar content being viewed by others
References
Huh, Y. M.; Jun, Y. W.; Song, H. T.; Kim, S.; Choi, J. S.; Lee, J. H.; Yoon, S.; Kim, K. S.; Shin, J. S.; Suh, J. S. et al. In vivo magnetic resonance detection of cancer by using multifunctional magnetic nanocrystals. J. Am. Chem. Soc. 2005, 127, 12387–12391.
Lee, J. H.; Huh, Y. M.; Jun, Y. W.; Seo, J. W.; Jang, J. T.; Song, H. T.; Kim, S.; Cho, E. J.; Yoon, H. G.; Suh, J. S. et al. Artificially engineered magnetic nanoparticles for ultra-sensitive molecular imaging. Nat. Med. 2007, 13, 95–99.
Osterfeld, S. J.; Yu, H.; Gaster, R. S.; Caramuta, S.; Xu, L.; Han, S.; Hall, D. A.; Wilson, R. J.; Sun, S.; White, R. L. et al. Multiplex protein assays based on real-time magnetic nanotag sensing. P. Natl. Acad. Sci. USA 2008, 105, 20637–20640.
Gaster, R. S.; Hall, D. A.; Nielsen, C. H.; Osterfeld, S. J.; Yu, H.; Mach, K. E.; Wilson, R. J.; Murmman, B.; Liao, J. C.; Gambhir, S. S. et al. Matrix-insensitive protein assays push the limits of biosensors in medicine. Nat. Med. 2009, 15, 1327–1332.
Gaster, R. S.; Xu, L.; Han, S. J.; Wilson, R. J.; Hall, D. A.; Osterfeld, S. J.; Yu, H.; Wang, S. X. Quantification of protein interactions and solution transport using high-density GMR sensor arrays. Nat. Nanotechnol. 2011, 6, 314–320.
Brzeska, M.; Justus, M.; Schotter, J.; Bruckl, H.; Rott, K.; Reiss, G. Development of magnetoresistive sensors for the detection of single molecules by magnetic markers. Molecular Phys. Rep. 2004, 39, 32–38.
Huang, H.; Delikanli, S.; Zeng, H.; Ferkey, D. M.; Pralle, A. Remote control of ion channels and neurons through magnetic-field heating of nanoparticles. Nat. Nanotechnol. 2010, 5, 602–606.
Kim, D. H.; Rozhkova, E. A.; Ulasov, I. V.; Bader, S. D.; Rajh, T.; Lesniak, M. S.; Novosad, V. Biofunctionalized magnetic-vortex microdiscs for targeted cancer-cell destruction. Nat. Mater. 2010, 9, 165–171.
Sniadecki, N. J.; Anguelouch, A.; Yang, M. T.; Lamb, C. M.; Liu, Z.; Kirschner, S. B.; Liu, Y.; Reich, D. H.; Chen, C. S. Magnetic microposts as an approach to apply forces to living cells. P. Natl. Acad. Sci. USA 2007, 104, 14553–14558.
Earhart, C. M.; Wilson, R. J.; White, R. L.; Pourmand, N.; Wang, S. X. Microfabricated magnetic sifter for high-throughput and high-gradient magnetic separation. J. Magn. Magn. Mater. 2009, 321, 1436–1439.
Sun, S.; Zeng, H.; Robinson, D. B.; Raoux, S.; Rice, P. M.; Wang, S. X.; Li, G. Monodisperse MFe2O4 (M = Fe, Co, Mn) nanoparticles. J. Am. Chem. Soc. 2004, 126, 273–279.
Hu, W.; Wilson, R. J.; Koh, A.; Fu, A.; Faranesh, A. Z.; Earhart, C. M.; Osterfeld, S. J.; Han, S.; Xu, L.; Guccione, S. et al. High-moment antiferromagnetic nanoparticles with tunable magnetic properties. Adv. Mater. 2008, 20, 1479–1483.
Hu, W.; Zhang, M. L.; Wilson, R. J.; Koh, A. L.; Wi, J. S.; Tang, M.; Sinclair, R.; Wang, S. X. Fabrication of planar, layered nanoparticles using tri-layer resist templates. Nanotechnology 2011, 22, 185302.
Wi, J. S.; Barnard, E. S.; Wilson, R. J.; Zhang, M. L.; Tang, M.; Brongersma, M. L.; Wang. S. X. Sombrero-shaped plasmonic nanoparticles with molecular-level sensitivity and multifunctionality. ACS Nano 2011, 5, 6449–6457.
Fu, A.; Hu, W.; Xu, L.; Wilson, R. J.; Yu, H.; Osterfeld, S. J.; Gambhir, S. S.; Wang, S. X. Protein-functionalized synthetic antiferromagnetic nanoparticles for biomolecule detection and magnetic manipulation. Angew. Chem. Int. Ed. 2009, 48, 1620–1624.
Zhang, M. L.; Hu, W.; Earhart, C. M.; Tang, M.; Wilson, R. J.; Wang, S. X. Silane-based functionalization of synthetic antiferromagnetic nanoparticles for biomedical applications. J. Appl. Phys. 2010, 107, 09B325.
Stöber, W.; Fink, A.; Bohn, E. Controlled growth of monodisperse silica spheres in the micron size range. J. Colloid Interf. Sci. 1968, 26, 62–69.
Kobayashi, Y.; Kakinuma, H.; Nagao, D.; Ando, Y.; Miyazaki, T.; Konno, M. Silica coating of Co-Pt alloy nanoparticles prepared in the presence of poly(vinylpyrrolidone). J. Nanopart. Res. 2009, 11, 1787–1794.
Kobayashi, Y.; Katakami, H.; Mine, E.; Nagao, D.; Konno, M.; Liz-Marzán, L. M. Silica coating of silver nanoparticles using a modified Stöber method. J. Colloid Interf. Sci. 2005, 283, 392–396.
Mine, E.; Yamada, A.; Kobayashi, Y.; Konno, M.; Liz-Marzán, L. M. Direct coating of gold nanoparticles with silica by a seeded polymerization technique. J. Colloid Interf. Sci. 2003, 264, 385–390.
Wilson, R. J.; Hu, W.; Fu, C. W. P.; Koh, A. L.; Gaster, R. S.; Earhart, C. M.; Fu, A. H.; Heilshorn, S. C.; Sinclair, R.; Wang, S. X. Formation and properties of magnetic chains for 100 nm nanoparticles used in separation of molecules and cells. J. Magn. Magn. Mater. 2009, 321, 1452–1458.
Joisten, H.; Courcier, T.; Balint, P.; Sabon, P.; Faure-Vincent, J.; Auffret, S.; Dieny, B. Self-polarization phenomenon and control of dispersion of synthetic antiferromagnetic nanoparticles for biological applications. Appl. Phys. Lett. 2010, 97, 253112.
Dreyfus, R.; Baudry, H.; Roper, M. L.; Fermigier, M.; Stone, H. A.; Bibette, J. Microscopic artificial swimmer. Nature 2005, 437, 862–865.
Ghosh, D.; Lee, Y.; Thomas, S.; Kohli, A. G.; Yun, D. S.; Belcher, A. M.; Kelly, K. A. M13-templated magnetic nanoparticles for targeted in vivo imaging of prostate cancer. Nat. Nanotechnol. 2012, 7, 677–682.
Ooi, C.; Earhart, C. M.; Wilson, R. J.; Wang, S. X. Effect of magnetic field gradient on effectiveness of the magnetic sifter for cell purification. IEEE T. Magn. 2013, 49, 316–320.
Johnston, B. M.; Johnston, P. R.; Corney, S.; Kilpatrick, D. Non-newtonian blood flow in human right coronary arteries: Steady state simulations. J. Biomech. 2004, 37, 709–720.
Yavuz, C. T.; Mayo, J. T.; Yu, W. W.; Prakash, A.; Falkner, J. C.; Yean, S.; Cong, L. L.; Shipley, H. J.; Kan, A.; Tomson, M. et al. Low-field magnetic separation of monodisperse Fe3O4 nanocrystals. Science 2006, 314, 964–967.
Author information
Authors and Affiliations
Corresponding author
Electronic supplementary material
Rights and permissions
About this article
Cite this article
Zhang, M., Earhart, C.M., Ooi, C. et al. Functionalization of high-moment magnetic nanodisks for cell manipulation and separation. Nano Res. 6, 745–751 (2013). https://doi.org/10.1007/s12274-013-0352-4
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12274-013-0352-4