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The protective mechanism of quercetin-3-O-β-d-glucuronopyranoside (QGC) in H2O2–induced injury of feline esophageal epithelial cells

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Abstract

Quercetin-3-O-β-d-glucuronopyranoside (QGC) is a flavonoid glucoside extracted from Rumex Aquaticus. Recent studies have shown that QGC exhibits anti-inflammatory, anti-oxidateve effect in vivo and cytoprotective effect in vitro. Reactive oxygen species (ROS), at low concentration, play role as a primary signal or second messenger, however, at high concentration, ROS are cytotoxic. In this study, we investigated the protective mechanism of QGC in H2O2–induced injury of Feline Esophageal Epithelial Cells. Primary-cultured feline esophagus cells were identified by an indirect immunofluorescent staining method using a cytokeratin monoclonal antibody. Cell viability was determined by the conventional MTT reduction assay. Western blot analysis was performed with specific antibodies to investigate the activation of MAPKs, NF-κB, and IκB-α, and the expression of COX-2. When the cells were exposed to 600 μM H2O2 medium for 24 h, cell viability decreased to 54 %. However, when cells were pretreated with 50–150 μM QGC for 12 h, the viability of cells exposed to H2O2 significantly increased in the dose dependent manner. QGC (50 μM, 12 h) also inhibited the expression of COX-2 induced by 10 μM H2O2 for 24 h. We found that treatment of H2O2 activated p38 MAPK and JNK, but not ERK. However QGC inhibited the H2O2-induced p38 MAPK and JNK phosphorylation. In addition, NF-κB was activated by H2O2 and translocated into the nucleus, but QGC inhibited the activation of NF-κB by blocking degradation of IκB. These data suggest that QGC reduces H2O2-induced COX-2 production by modulating the p38 MAPK, JNK, NF-κB signal pathway in feline esophageal epithelial cells.

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Acknowledgments

This research was partially supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (2011-0012139) and Chung-Ang University Research Scholarship Grants in 2015–2016.

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    1. Department of Pharmacology, College of Pharmacy, Chung-Ang University, Seoul, 156-756, Republic of Korea

      Uy Soo Sohn, Se Eun Lee, Sung Hee Lee, Yoonjin Nam & Uy Dong Sohn

    2. Department of Pharmaceutical botany, College of Pharmacy, Chung-Ang University, Seoul, 156-756, Republic of Korea

      Wan Kyunn Hwang

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    Uy Soo Sohn and Se Eun Lee have contributed equally to this work.

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    Sohn, U.S., Lee, S.E., Lee, S.H. et al. The protective mechanism of quercetin-3-O-β-d-glucuronopyranoside (QGC) in H2O2–induced injury of feline esophageal epithelial cells. Arch. Pharm. Res. 39, 1324–1334 (2016). https://doi.org/10.1007/s12272-016-0808-7

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