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EPA attenuates ultraviolet radiation-induced downregulation of aquaporin-3 in human keratinocytes

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Abstract

Eicosapentaenoic acid (EPA) is an omega-3 polyunsaturated fatty acid (ω-3 PUFA) that protects against photodamage and photocarcinogenesis in mammals. Aquaporin-3 (AQP3) is a water/glycerol transport protein that is found in basal layer keratinocytes. In this study, we have investigated the protective effect of EPA against ultraviolet B (UVB)-induced AQP3 downregulation in human keratinocytes. EPA treatment was found to increase AQP3 gene and protein expression in human epidermal keratinocytes (HaCaT). Using a specific inhibitor, we observed that the effect of EPA on AQP3 expression was mediated by extracellular signal-regulated kinase (ERK) activation. UVB radiation induced AQP3 downregulation in HaCaT cells, and it was found that EPA treatment attenuated UVB-induced AQP3 reduction and the associated cell death. UVB-induced downregulation of AQP3 was blocked by EPA and p38 inhibitor SB203580. Collectively, the present results show that EPA increased AQP3 expression and that this led to a reduction UVB-induced photodamage.

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Acknowledgments

This work was supported by a Grant of the Korea Healthcare technology R&D Project, Ministry of Health & Welfare [No. 103017] and by the National Research Foundation of Korea grant funded by the Korea government [No. 2008-0062484].

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Authors and Affiliations

  1. BK21-plus Team, Professional Graduate School of Oriental Medicine, Wonkwang University, Iksan, 570-749, Jeollabukdo, Republic of Korea

    Byoung-Kook Jeon, Ho-Sub Lee & Yeun-Ja Mun

  2. Department of Anatomy, College of Oriental Medicine, Wonkwang University, Iksan, 570-749, Korea

    Moon-Kyung Kang & Won-Hong Woo

  3. Coreana Cosmetics, R&D Center, Cheonan, 330-833, Korea

    Ghang-Tai Lee & Kun-Kuk Lee

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  1. Byoung-Kook Jeon
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  2. Moon-Kyung Kang
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Correspondence to Yeun-Ja Mun.

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Jeon, BK., Kang, MK., Lee, GT. et al. EPA attenuates ultraviolet radiation-induced downregulation of aquaporin-3 in human keratinocytes. Arch. Pharm. Res. 38, 1552–1560 (2015). https://doi.org/10.1007/s12272-014-0482-6

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  • DOI: https://doi.org/10.1007/s12272-014-0482-6

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