Skip to main content
SpringerLink
Log in

Reversibel schaltbare fluoreszierende Proteine für die Superauflösung

  • Nanoskopie
  • Published:
BIOspektrum Aims and scope

Abstract

Super-resolution microscopy, or nanoscopy, enables the visualization of cellular structures inaccessible to conventional light microscopy. RESOLFT nanoscopy is especially suitable for the imaging of living cells. It requires fluorescent proteins (RSFPs) that can be repeatedly switched between a fluorescent and a non-fluorescent state by light. We have analyzed the molecular switching mechanisms and generated a family of RSFPs specifically tailored for live cell RESOLFT nanoscopy.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Literatur

  1. Tsien RY (1998) The green fluorescent protein. Annu Rev Biochem 67:509–544

    Article  CAS  PubMed  Google Scholar 

  2. Hell SW (2009) Microscopy and its focal switch. Nat Methods 6:24–32

    Article  CAS  PubMed  Google Scholar 

  3. Andresen M, Wahl MC, Stiel AC et al. (2005) Structure and mechanism of the reversible photoswitch of a fluorescent protein. Proc Natl Acad Sci USA 102:13070–13074

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Andresen M, Stiel AC, Trowitzsch S et al. (2007) Structural basis for reversible photoswitching in Dronpa. Proc Natl Acad Sci USA 104:13005–13009

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. Brakemann T, Stiel AC, Weber G et al. (2011) A reversibly photoswitchable GFP-like protein with fluorescence excitation decoupled from switching. Nat Biotechnol 29:942–947

    Article  CAS  PubMed  Google Scholar 

  6. Grotjohann T, Testa I, Reuss M et al. (2012) rsEGFP2 enables fast RESOLFT nanoscopy of living cells. eLife 1:e00248

  7. Grotjohann T, Testa I, Leutenegger M et al. (2011) Diffraction-unlimited all optical imaging and writing with a photochromic GFP. Nature 478:204–208

    Article  CAS  PubMed  Google Scholar 

  8. Testa I, D’Este E, Urban NT et al. (2015) Dual channel RESOLFT nanoscopy by using fluorescent state kinetics. Nano Lett 15:103–106

    Article  CAS  PubMed  Google Scholar 

  9. Lavoie-Cardinal F, Jensen NA, Westphal V et al. (2014) Two-color RESOLFT nanoscopy with green and red fluorescent photochromic proteins. Chemphyschem 15:655–663

    Article  CAS  PubMed  Google Scholar 

  10. Ratz M, Testa I, Hell SW et al. (2015) CRISPR/Cas9-mediated endogenous protein tagging for RESOLFT super-resolution microscopy of living human cells. Sci Rep 5:9592

    Article  CAS  PubMed  PubMed Central  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Abteilung NanoBiophotonik, Max-Planck-Institut für biophysikalische Chemie, Am Fassberg 11, D-37077, Gottingen, Germany

    Martin Andresen, Nickels Jensen & Stefan Jakobs

Authors
  1. Martin Andresen
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Nickels Jensen
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Stefan Jakobs
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Stefan Jakobs.

Additional information

Martin Andresen 1998–2004 Biologiestudium an der Universität Göttingen, dort 2004–2009 Promotion. Seit 2009 Postdoc am Max-Planck-Institut für bio - physikalische Chemie, Göttingen.

Nickels Jensen 1999–2005 Biologiestudium an den Universitäten Kiel und Bielefeld. 2005–2010 Promotion an der Universität Bielefeld. Seit 2010 Postdoc am Max-Planck-Institut für biophysikalische Chemie, Göttingen.

Stefan Jakobs 1990–1995 Biologiestudium an den Universitäten Kaiserslautern und Manchester, UK. 1995–1999 Promotion am Max-Planck-Institut für Züchtungsforschung, Köln. 2007 Habilitation (Zellbiologie/Botanik) an der Universität Göttingen. Postdocs in Köln und Göttingen in der Abteilung von Prof. Dr. S. W. Hell. Seit 2005 Forschungsgruppenleiter am Max-Planck-Institut für biophysikalische Chemie, Göttingen. Seit 2010 Professor für hochauflösende Mikroskopie an der Universitätsmedizin Göttingen.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Andresen, M., Jensen, N. & Jakobs, S. Reversibel schaltbare fluoreszierende Proteine für die Superauflösung. Biospektrum 22, 365–367 (2016). https://doi.org/10.1007/s12268-016-0699-7

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12268-016-0699-7

Search

Navigation