Abstract
The DNA-array to protein-array technology (DAPA) allows the direct transcription and translation of a coded DNA-array to a protein array in the presence of a cell free expression system. The coupling efficiency of DNA and of the corresponding immobilized proteins is enhanced by using 3-dimensional epoxy surfaces. The production time of protein arrays is considerably reduced and the DNA template array can be reused for producing further protein arrays.
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Literatur
Stoevesandt O, Taussig MJ, He M (2009) Protein microarrays: high throughput tools for proteomics. Expert Rev Proteomics 6:145–157
Jeong JS, Jiang L, Albino E et al. (2012) Rapid identification of monospecific monoclonal antibodies using a human proteome microarray. Mol Cell Proteomics 11:O111.016253
Hu CJ, Song G, Huang W et al. (2012) Identification of new autoantigens for primary biliary cirrhosis using human proteome microarrays. Mol Cell Proteomics 11:669–680
He M, et al. (2008) Printing protein arrays from DNA arrays. Nat Methods 5:175–177
Stoevesandt O (2012) Protein arraying by cell-free expression and diffusion across a fluid-filled gap. N Biotechnol 29:586–588
Schmidt R, et al. (2013) Optimised ‘on demand’ protein arraying from DNA by cell free expression with the ‘DNA to Protein Array’ (DAPA) technology. J Proteomics 88:141–148
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Stoevesandt, O., Schmidt, R., Heise, C. et al. 3D-Epoxyoberflächen für DNA-codierte Proteinarrays. Biospektrum 20, 453–455 (2014). https://doi.org/10.1007/s12268-014-0461-y
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DOI: https://doi.org/10.1007/s12268-014-0461-y