Abstract
Zostera asiatica is one of the five members of the genus Zostera that can be found in Korea. Studies have reported the phytochemical properties and bioactivities of Zostera species. Current study focused on the antioxidant effects of Z. asiatica as a part of ongoing research for bioactive substances from marine resources. Results indicated that a crude extract of Z. asiatica not only scavenged on peroxynitrite in vitro and on intracellular reactive oxygen species (ROS) but also inhibited production of nitric oxide (NO). The crude extract was subjected to solvent fractionation for bioactivity-based separation using aforementioned three bioassay systems. From the active n-butanol fraction, two flavonoids were isolated and characterized as luteolin (1) and luteolin-3’-sulfate (2). Both flavonoids showed significant antioxidant effects. In conclusion, Z. asiatica was demonstrated to possess antioxidant effect partly attributed to isolated flavonoids, the first such effect reported from Z. asiatica, to the best of our knowledge.
Similar content being viewed by others
References
Berlett, B. S. and E. R. Stadtman (1997) Protein oxidation in aging, disease, and oxidative stress. J. Biol. Chem. 272: 20313–20316.
Mayne S. T. (2003) Antioxidant nutrients and chronic disease: Use of biomarkers of exposure and oxidative stress status in epidemiologic research. J. Nutr. 133: 933S-940S.
Elmarakby, A. A. and J. C. Sullivan (2012) Relationship between oxidative stress and inflammatory cytokines in diabetic nephropathy. Cardiovasc. Ther. 30: 49–59.
Holvoet, P. (2008) Relations between metabolic syndrome, oxidative stress and inflammation and cardiovascular disease. Verh. K. Acad. Geneeskd. Belg. 70: 193–219.
Khansari, N., Y. Shakiba, and M. Mahmoudi (2009) Chronic inflammation and oxidative stress as a major cause of age-related diseases and cancer. Recent Pat. Inflamm. Allergy Drug Discov. 3: 73–80.
Cadenas, E. and K. J. A. Davies (2000) Mitochondrial free radical generation, oxidative stress, and aging. Free Radical Biol. Med. 29: 222–230.
Conner, E. M. and M. B. Grisham (1996) Inflammation, free radicals, and antioxidants. Nutrition 12: 274–277.
Fylaktakidou, K. C., D. J. Hadjipavlou-Litina, K. E. Litinas, and D. N. Nicolaides (2004) Natural and synthetic coumarin derivatives with anti-inflammatory/antioxidant activities. Curr. Pharm. Des. 10: 3813–3833.
Geronikaki, A. A. and A. M. Gavalas (2006) Antioxidants and inflammatory disease: Synthetic and natural antioxidants with anti-inflammatory activity. Comb. Chem. High Throughput Screen. 9: 425–442.
Nichols, J. and S. Katiyar (2010) Skin photoprotection by natural polyphenols: Anti-inflammatory, antioxidant and DNA repair mechanisms. Arch. Dermatol. Res. 302: 71–83.
Shin, H. and H. K. Choi (1998) Taxonomy and distribution of Zostera (Zosteraceae) in eastern Asia, with special reference to Korea. Aquat. Bot. 60: 49–66.
Ok, J. S., S. Y. Lee, K. H. Shin, and H. J. Kim (2013) Seasonal variation characteristics of Zostera marina L. in Haenam Sagumi on the southern coast of Korea. Kor. J. Lomnol. 46: 513–523.
Harborne, J. B. and C. A. Williams (1976) Occurrence of sulphated flavones and caffeic acid esters in members of the fluviales. Biochem. Syst. Ecol. 4: 37–41.
Quackenbush, R. C., D. Bunn, and W. Lingren (1986) HPLC determination of phenolic acids in the water-soluble extract of Zostera marina L. (eelgrass). Aquat. Bot. 24: 83–89.
Vergeer, L. H. T. and A. Develi (1997) Phenolic acids in healthy and infected leaves of Zostera marina and their growth-limiting properties towards Labyrinthula zosterae. Aquat. Bot. 58: 65–72.
Buchsbaum, R. N., F. T. Short, and D. P. Cheney (1990) Phenolic-nitrogen interactions in eelgrass, Zostera marina L.: Possible implications for disease resistance. Aquat. Bot. 37: 291–297.
Todd, J. S., R. C. Zimmerman, P. Crews, and R. S. Alberte (1993) The antifouling activity of natural and synthetic phenol acid sulphate esters. Phytochem. 34: 401–404.
Achamlale, S., B. Rezzonico, and M. Grignon-Dubois (2009) Evaluation of Zostera detritus as a potential new source of zosteric acid. J. Appl. Phycol. 21: 347–352.
Wang, J., X. Pan, Y. Han, D. Guo, Q. Guo, and R. Li (2012) Rosmarinic acid from eelgrass shows nematicidal and antibacterial activities against pine wood nematode and its carrying bacteria. Mar. Drugs. 10: 2729–2740.
Okimoto, Y., A. Watanabe, E. Niki, T. Yamashita, and N. Noguchi (2000) A novel fluorescent probe diphenyl-1-pyrenylphosphine to follow lipid peroxidation in cell membranes. FEBS Lett. 474: 137–140.
Green, L. C., D. A. Wagner, J. Glogowski, P. L. Skipper, J. S. Wishnok, and S. R. Tannenbaum. Analysis of nitrate, nitrite, and [15N]nitrate in biological fluids. Anal. Biochem. 126: 131-138.
Kooy, N., J. Royall, H. Ischiropoulos, and J. Beckman (1994) Peroxynitrite-mediated oxidation of dihydrorhodamine 123. Free Radic. Biol. Med. 16: 149–156.
Yoshioka, T., T. Inokuchi, S. Fujioka, and Y. Kimura (2004) Phenolic compounds and flavonoids as plant growth regulators from fruit and leaf of Vitex rotundifolia. Z. Naturforsch C. 59: 509–514.
Loizzo, M. R., A. Said, R. Tundis, K. Rashed, G. A. Statti, A. Hufner, and F. Menichini (2007) Inhibition of angiotensin converting enzyme (ACE) by flavonoids isolated from Ailanthus excelsa (Roxb) (Simaroubaceae). Phyther. Res. 21: 32–36.
Seabra, R. M. and A. C. Alves (1991) Quercetin 3′-Sulphate from Hypericum elodes. Phytochem. 30: 1344–1345.
Williams, C. A., J. B. Harborne, and T. S. Crosby (1976) Tricetin, diosmetin and luteolin sulphates in leaves of Lachenalia unifolia. Phytochem. 15: 349–350.
Regalado, E. L., R. Menendez, O. Valdés, R. A. Morales, A. Laguna, C. P. Thomas, Y. Hernandez, C. Nogueiras, and A. Kijjoa (2012) Phytochemical analysis and antioxidant capacity of BM-21, a bioactive extract rich in polyphenolic metabolites from the sea grass Thalassia testudinum. Nat. Prod. Commun. 7: 47–50.
Laskin, D. L. and K. J. Pendino (1995) Macrophages and inflammatory mediators in tissue injury. Annu. Rev. Pharmacol. Toxicol. 35: 655–677.
Virág, L., E. Szabó, P. Gergely, and C. Szabó (2003) Peroxynitrite-induced cytotoxicity: Mechanism and opportunities for intervention. Toxicol. Lett. 140: 113–124.
Hyun, S. K., H. A. Jung, H. Y. Chung, and J. S. Choi (2006) In vitro peroxynitrite scavenging activity of 6-hydroxykynurenic acid and other flavonoids from Gingko biloba yellow leaves. Arch. Pharm. Res. 29: 1074–1079.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Kim, H., Jeong, H., Hong, J.W. et al. Evaluation of flavonoids from Zostera asiatica as antioxidants and nitric oxide inhibitors. Biotechnol Bioproc E 21, 823–829 (2016). https://doi.org/10.1007/s12257-016-0567-9
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12257-016-0567-9