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Drosophila melanogaster S2 cells are more suitable for the production of recombinant COX-1 than Trichoplusia ni BTI TN-5B1-4 cells

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Abstract

Recombinant human cyclooxygenase-1 (COX-1) was expressed from stably transfected Trichoplusia ni BTI TN-5B1-4 (TN-5B1-4) and Drosophila melanogaster S2 cells. Two kinds of recombinant COX-1 with molecular weights (MWs) of 68 and 74 kDa were expressed in the intracellular fractions of stably transfected TN-5B1-4/ COX-1 and S2/COX-1 cells, due to glycosylation. The recombinant COX-1 secreted to medium fractions has a MW of 72 kDa. Recombinant COX-1 in the intracellular fractions was purified to homogeneity using a one-step Ni-NTA affinity fractionation method. Recombinant COX-1 purified from TN-5B1-4/COX-1 and S2/COX-1 cells contained 11,389 and 33,850 Unit/mg of specific peroxidase activity, respectively. The maximum productions of intracellular recombinant COX-1 were 1.7 and 5.6 μg/107 cells in the T-flask cultures of TN-5B1-4/COX-1 and S2/COX-1 cells, respectively. Taken together, our findings indicate that S2 cells can be more suitable system to produce recombinant COX-1, compared to TN-5B1-4 cells.

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Authors and Affiliations

  1. Department of Genetic Engineering and Graduate School of Biotechnology, Kyung Hee University, Yongin, 446-701, Korea

    Kyung Hwa Chang, Jong-Hwa Park, Jeon Hwang-Bo, Dae Kyun Chung & In Sik Chung

  2. RNA Inc., Yongin, 446-701, Korea

    Dae Kyun Chung

  3. Department of Microbiology and Research Center for Medical Sciences, Chung-Ang University College of Medicine, Seoul, 156-756, Korea

    Wonyong Kim

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  1. Kyung Hwa Chang
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Correspondence to In Sik Chung.

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Chang, K.H., Park, JH., Hwang-Bo, J. et al. Drosophila melanogaster S2 cells are more suitable for the production of recombinant COX-1 than Trichoplusia ni BTI TN-5B1-4 cells. Biotechnol Bioproc E 19, 803–810 (2014). https://doi.org/10.1007/s12257-014-0239-6

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