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A new microtiter plate-based screening method for microorganisms producing Alpha-amylase inhibitors

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Abstract

Alpha-amylase inhibitors are widely used by the pharmaceutical and agricultural industries, such as the treatment of diabetes and obesity and insect controller. Here, we developed a colorimetric method to screen for α-amylase inhibitor producing strains or mutants with higher α-amylase inhibitor productivity. This method relies on absorbance changes at 402 nm that are due to the inhibition of α-amylase catalyzed hydrolysis of 2-Chloro-4-nitrophenyl-4-O-β-D-galactopyranosyl-maltoside by α-amylase inhibitors. The assay can be performed on a microtiter plate, making it simple and convenient. Using this method, α-amylase inhibitor producing strains and mutants with higher α-amylase inhibitor productivity can be rapidly screened. One strain, ZJB-08196, with the highest α-amylase inhibition was isolated and identified as Actinoplanes utahensis, and one mutant with higher acarbose production was obtained by screening 3,000 variants using this method.

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Authors and Affiliations

  1. Institute of Bioengineering, Zhejiang University of Technology, Zhejiang, 310-014, China

    Zhi-Hua Feng, Yuan-Shan Wang & Yu-Guo Zheng

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  1. Zhi-Hua Feng
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  2. Yuan-Shan Wang
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  3. Yu-Guo Zheng
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Correspondence to Yu-Guo Zheng.

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Feng, ZH., Wang, YS. & Zheng, YG. A new microtiter plate-based screening method for microorganisms producing Alpha-amylase inhibitors. Biotechnol Bioproc E 16, 894–900 (2011). https://doi.org/10.1007/s12257-011-0033-7

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  • DOI: https://doi.org/10.1007/s12257-011-0033-7

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