Abstract
The purpose of this study is to detect the clinical significance of NDRG1 and its relationship with MMP-9 in gastric cancer metastatic progression. 101 cases of gastric cancer specimens were utilized to identify the protein expression of NDRG1 and MMP-9 by immunohistochemistry, their clinical significance was also analyzed. The suppression by siRNA-NDRG1 was employed to detect the role of NDRG1 in gastric cancer progression and its relationship with MMP-9. NDRG1 expression was correlated inversely with the degree of tumor cell differentiation (p < 0.01), invasion depth (p < 0.05), lymph node metastasis (p < 0.05) and TNM stage (p < 0.05), whereas MMP-9 was positive correlated with the degree of tumor cell differentiation (p < 0.01), lymph node metastasis (p < 0.05) and TNM stage (p < 0.05), but not correlated with invasion depth (p>0.05). Furthermore, cell proliferation and invasion effect were remarkably enhanced when NDRG1 was silencing, but MMP-9 expression was increased. NDRG1 silencing enhances gastric cancer cells progression through upregulating MMP-9. It suggests that NDRG1 may inhibit the metastasis of gastric cancer via regulating MMP-9.
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Acknowledgments
This work was supported in part by a grant from the National Natural Science Foundation of China (#30572162), Higher Specialized Research Fund for Doctoral Program of Ministry of Education of China (#20102104110001) and the Natural Science Foundation of Hebei Province (#H2015206344). The authors would like to thank Institute of General Surgery of China Medical University for supports.
Authors’ Contributions
Xiaojing Chang carried out the molecular genetic studies and drafted the manuscript. Xiaoyang Xu carried out the Western blotting. Jinguo Ma participated in the sequence alignment. Zhenhua Li carried out the IHC, Peng Deng and Jing Chen participated in Cell culture and transfection. Shuanglong Zhang carried out the Alamar Blue. Xiaoying Xue and Yu Zhi participated in the design of the study and performed the statistical analysis. Dongqiu Dai participated in its design and coordination and helped to draft the manuscript. All authors read and approved the final manuscript.
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Chang, X., Xu, X., Xue, X. et al. NDRG1 Controls Gastric Cancer Migration and Invasion through Regulating MMP-9. Pathol. Oncol. Res. 22, 789–796 (2016). https://doi.org/10.1007/s12253-016-0071-8
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DOI: https://doi.org/10.1007/s12253-016-0071-8