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Effects of Mesalazine on Morphological and Functional Changes in the Indomethacin-Induced Inflammatory Bowel Disease (Rat Model of Crohn’s Disease)

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Pathology & Oncology Research

Abstract

Morphological and functional changes have been investigated in the rat model of Crohn’s disease. The inflammatory bowel disease was induced by indomethacin (1 × 10 mg/kg s.c. for 3 days). Morphological alterations were evaluated by macroscopic scoring system and on the base of histological changes in the small intestine. Functional activities were studied by determination of the intestinal and hepatic elimination of p-Nitrophenol (PNP) and its metabolites (PNP-glucuronide: PNP-G and PNP-sulfate: PNP-S) during the luminal perfusion of PNP. It was found that the indomethacin induced severe macroscopic changes (hyperaemia, petechia, bleeding, erosions, ulcerations) and significant histological alterations in the small intestine of rats which were definitely inhibited by mesalazine (1000 mg/kg by gastric tube for 3 days). Disappearance of PNP from the luminal perfusion solution was diminished by indomethacin which was corrected by administration of mesalazine. Significant depression was found in the luminal appearance of PNP metabolites by giving of indomethacin and these alterations could not be compensated by mesalazine.

Hepatic elimination of PNP (biliary excretion of PNP and its metabolites) was decreased definitely by indomethacin which was – at least partly - compensated by mesalazine.

The findings of the present study suggest that the indomethacin-induced inflammation in the small intestine represents a useful rat model of Crohn’s disease. Morphological and functional alterations caused by indomethacin can be compensated by mesalazine.

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Authors and Affiliations

  1. Department of Pharmacology and Pharmacotherapy, Medical Faculty, University of Pécs, 12 Szigeti út, Pécs, Hungary

    Higin Simon, Tamás Fischer & Emil Fischer

  2. Department of Pharmaceutical Chemistry, Pharmaceutical Faculty, University of Pécs, 2 Rókus utca, Pécs, Hungary

    Attila Almási

Authors
  1. Higin Simon
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  2. Tamás Fischer
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  3. Attila Almási
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  4. Emil Fischer
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Correspondence to Emil Fischer.

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All procedures were carried out on animals according to the Hungarian Animals Act (Scientific Procedures, 1998), and the study was approved by the Ethics Committee on Animal Research of the University of Pécs.

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The authors declare that they have no conflict of interest.

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Simon, H., Fischer, T., Almási, A. et al. Effects of Mesalazine on Morphological and Functional Changes in the Indomethacin-Induced Inflammatory Bowel Disease (Rat Model of Crohn’s Disease). Pathol. Oncol. Res. 23, 41–46 (2017). https://doi.org/10.1007/s12253-016-0069-2

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  • DOI: https://doi.org/10.1007/s12253-016-0069-2

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