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Anti Genotoxic Effect of Mosinone-A on 7, 12-Dimethyl Benz[a] Anthracene Induced Genotoxicity in Male Golden Syrian Hamsters

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Pathology & Oncology Research

Abstract

The present study was aimed to evaluate the antigenotoxic effect of Mosinone-A on 7,12-dimethylbenz[a]anthracene induced genotoxicity. The frequency of micronucleated polychromatic erythrocytes [MnPCEs], chromosomal aberrations [CA], DNA damage (comet assay) as cytogenetic markers and the status of lipid peroxidation byproducts, antioxidants and phase II detoxification agents were used as biochemical markers to assess the antigenotoxic effect of Mosinone-A on DMBA induced genotoxicity. A single intraperitoneal injection of DMBA (30 mg/kg b.wt) to golden Syrian hamsters, resulted in marked elevation in the frequency of MnPCEs, aberrations in the chromosomal structure were found in bone marrow and DNA damage (comet assay) was found in blood cells and altered level of lipid peroxidation, antioxidants, and phase II detoxification agents. Oral pretreatment of Mosinone-A (2 mg/kg b.wt) for 5 days to DMBA treated animals significantly reduced the frequency of MnPCEs, chromosomal abnormalities such as chromosomal break, gap, minute, fragment, DNA damage and reversed the status of biochemical variables. Our results thus demonstrated the antigenotoxic effect of Mosinone-A on DMBA induced genotoxicity in male golden Syrian hamsters.

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Abbreviations

DMBA:

7, 12-dimethylbenz[a]anthracene

MnPCEs:

Micronucleated polychromatic erythrocytes

NCEs:

Normochromatic erythrocytes

PCEs:

Polychromatic erythrocytes

CA:

Chromosomal aberrations

CAT:

Catalase

GPx:

Glutathione peroxidase

SOD:

Superoxide dismutase

GR:

Glutathione reductase

GST:

Glutathione-S-transferase

GSH:

Reduced glutathione

TBARS:

Thiobarbituric acid reactive substances

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Acknowledgement

Financial assistance from the Department of Science and Technology (DST) New Delhi, India is gratefully acknowledged.

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Authors and Affiliations

  1. Department of Biochemistry & Biotechnology, Faculty of Science, Annamalai University, Annamalai Nagar, Tamil Nadu, 608 002, India

    Govindasamy Sugunadevi, Kathiresan Suresh, Mariadoss Arokia Vijayaanand, Kasinathan Rajalingam & Jagadeesan Sathiyapriya

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  1. Govindasamy Sugunadevi
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  2. Kathiresan Suresh
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  3. Mariadoss Arokia Vijayaanand
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Correspondence to Kathiresan Suresh.

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Sugunadevi, G., Suresh, K., Vijayaanand, M.A. et al. Anti Genotoxic Effect of Mosinone-A on 7, 12-Dimethyl Benz[a] Anthracene Induced Genotoxicity in Male Golden Syrian Hamsters. Pathol. Oncol. Res. 18, 69–77 (2012). https://doi.org/10.1007/s12253-011-9418-3

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