Abstract
The main goal of this study was the synthesis of copper oxide nanoparticles with a green method using Starch extracted from Solanum tuberosum. The synthesized copper oxide nanoparticles were characterized by different techniques, including X-ray diffraction (XRD), UV–Vis spectrophotometry, and scanning electron microscopy (SEM). All the analyses exposed that the nanoparticles were highly stable and spherical with mean size about 54 nm. The potential antibacterial activity of the copper oxide nanoparticles synthesized in the eco-friendly approach was tested against some standard strains of human pathogens (Gram-positive and -negative bacteria). Efficient antibacterial activities against Bacillus cereus 9634, Shigella sonnei, Staphylococcus epidermidis 1435, Enterococcus 1237, Pseudomonas aeruginosa PAO1 27853 and Escherichia coli 25922 and no significant antibacterial activity against Staphylococcus aureus 2592 were observed. Moreover, MTT assay demonstrated the anticancer activity ofthat the synthesized CuO nanoparticles against MCF-7 cell line.
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Alishah H, Pourseyedi S, Ebrahimipour SY, Esmaeili-Mahani S (2016) A green approach for the synthesis of silver nanoparticles using root extract of chelidonium majus: characterization and antibacterial evaluation. J Clust Sci 27:421–429
Ankamwar B (2010) Biosynthesis of gold nanoparticles (Green-Gold) using leaf extract of terminalia catappa. J Chem 7:1334–1339
Azam A, Ahmed AS, Oves M, Khan MS, Memic A (2012) Size-dependent antimicrobial properties of CuO nanoparticles against Gram-positive and -negative bacterial strains. Int J Nanomed 7:3527–3535
Bhushan B (2005) Micro/nanotribology and materials characterization studies using scanning probe microscopy. In: Nanotribology and nanomechanics. Springer Berlin Heidelberg, pp 315–387
Chen J, Wang K, Hartman L, Zhou W (2008) H2S detection by vertically aligned CuO nanowire array sensors. J Phys Chem C 112:16017–16021
Cioffi N, Ditaranto N, Torsi L, Picca R, Sabbatini L, Valentini A, Novello L, Tantillo G, Bleve-Zacheo T, Zambonin P (2005) Analytical characterization of bioactive fluoropolymer ultra-thin coatings modified by copper nanoparticles. Anal Bioanal Chem 381:607–616
Cuenya BR (2010) Synthesis and catalytic properties of metal nanoparticles: size, shape, support, composition, and oxidation state effects. Thin Solid Films 518:3127–3150
Dang TMD, Le TTT, Fribourg-Blanc E, Dang MC (2011) Synthesis and optical properties of copper nanoparticles prepared by a chemical reduction method. Adv Nat Sci Nanosci Nanotechnol 2:015009
Díaz C, Valenzuela M, Lavayen V, Mendoza K, Pena D, O'Dwyer C (2011) Nanostructured copper oxides and phosphates from a new solid-state route. Inorg Chim Acta 377:5–13
Doria G, Conde J, Veigas B, Giestas L, Almeida C, Assuncao M, Rosa J, Baptista PV (2012) Noble metal nanoparticles for biosensing applications. Sensors 12:1657–1687
Emanuela F, Marco T, Tiziana S, Daniela C, Cosimo M, Rachele GM (2014) Room temperature facile synthesis of CuO nanostructures by resistive heating. Physica E 60:59–64
Gréget R, Nealon GL, Vileno B, Turek P, Mény C, Ott F, Derory A, Voirin E, Rivière E, Rogalev A, Wilhelm F, Joly L, Knafo W, Ballon G, Terazzi E, Kappler JP, Donnio B, Gallani JL (2012) Magnetic properties of gold nanoparticles: a room-temperature quantum effect. Chem Phys Chem 13:3092–3097
Gunalan S, Sivaraj R, Venckatesh R (2012) Aloe barbadensis Miller mediated green synthesis of mono-disperse copper oxide nanoparticles: optical properties. Spectrochim Acta Part A 97:1140–1144
Hsieh C-T, Chen J-M, Lin H-H, Shih H-C (2003) Synthesis of well-ordered CuO nanofibers by a self-catalytic growth mechanism. Appl Phys Lett 82:3316–3318
Iravani S (2011) Green synthesis of metal nanoparticles using plants. Green Chem 13:2638–2650
Janssen LP (2009) Thermoplastic starch: a green material for various industries 119–148
Kamyshny A, Steinke J, Magdassi S (2011) Metal-based inkjet inks for printed electronics. Open Appl Phys J 4:19–36
Kandasamy K, Alikunhi NM, Manickaswami G, Nabikhan A, Ayyavu G (2013) Synthesis of silver nanoparticles by coastal plant Prosopis chilensis (L.) and their efficacy in controlling vibriosis in shrimp Penaeus monodon. Appl Nanosci 3:65–73
Kanhed P, Birla S, Gaikwad S, Gade A, Seabra AB, Rubilar O, Duran N, Rai M (2014) In vitro antifungal efficacy of copper nanoparticles against selected crop pathogenic fungi. Mater Lett 115:13–17
Kong M, Zhang W, Yang Z, Weng S, Chen Z (2011) Facile synthesis of CuO hollow nanospheres assembled by nanoparticles and their electrochemical performance. Appl Surf Sci 258:1317–1321
Kumar CS (2013) UV-vis and photoluminescence spectroscopy for nanomaterials characterization. Springer
Letfullin RR, Iversen CB, George TF (2011) Modeling nanophotothermal therapy: kinetics of thermal ablation of healthy and cancerous cell organelles and gold nanoparticles. Nanomed Nanotechnol Biol Med 7:137–145
Li H, Liao J, Zeng T (2014) A facile synthesis of CuO nanowires and nanorods, and their catalytic activity in the oxidative degradation of Rhodamine B with hydrogen peroxide. Catal Commun 46:169–173
Marshall AT, Haverkamp RG, Davies CE, Parsons JG, Gardea- Torresdey JL, van Agterveld D (2007) Accumulation of gold nanoparticles in Brassic juncea. Int J Phytorem 9:197–206
Meshram S, Adhyapak P, Mulik U, Amalnerkar D (2012) Facile synthesis of CuO nanomorphs and their morphology dependent sunlight driven photocatalytic properties. Chem Eng J 204:158–168
Mnyusiwalla A, Daar AS, Singer PA (2003) 'Mind the gap': science and ethics in nanotechnology. Nanotechnology 14:R9–R13
Mulvaney P (1996) Surface plasmon spectroscopy of nanosized metal particles. Langmuir 12:788–800
Nair B, Pradeep T (2002) Coalescence of nanoclusters and formation of submicron crystallites assisted by lactobacillus strains. Cryst Growth Des 2:293–298
Pacioni NL, Pardoe A, McGilvray KL, Chretien MN, Scaiano JC (2010) Synthesis of copper nanoparticles mediated by photogenerated free radicals: catalytic role of chloride anions. Photochem Photobiol Sci 9:766–774
Palacios-Hernandez T, Hirata-Flores GA, Contreras-Lopez OE, Mendoza-Sanchez ME, Valeriano-Arreola I, Gonzalez-Vergara E, Mendez-Rojas MA (2012) Synthesis of Cu and Co metal oxide nanoparticles from thermal decomposition of tartrate complexes. Inorg Chim Acta 392:277–282
Patterson A (1939) The scherrer formula for X-ray particle size determination. Phys Rev 56:978
Prabhavathi SP, Punitha J, Rajam PS, Ranjith R, Suresh G, Mala N, Maruthamuthu D (2014) Simple methods of synthesis of copper oxide, zinc oxide, lead oxide and barium oxide nanoparticles. J Chem Pharm Res 6:1472–1478
Ren G, Hu D, Cheng EW, Vargas-Reus MA, Reip P, Allaker RP (2009) Characterisation of copper oxide nanoparticles for antimicrobial applications. Int J Antimicrob Agents 33:587–590
Saison C, Perreault F, Daigle J-C, Fortin C, Claverie J, Morin M, Popovic R (2010) Effect of core-shell copper oxide nanoparticles on cell culture morphology and photosynthesis (photosystem II energy distribution) in the green alga, Chlamydomonas reinhardtii. Aquat Toxicol 96:109–114
Sankar R, Maheswari R, Karthik S, Shivashangari KS, Ravikumar V (2014) Anticancer activity of Ficus religiosa engineered copper oxide nanoparticles. Mater Sci Eng C 44:234–239
Schultz S, Smith DR, Mock JJ, Schultz DA (2000) Single-target molecule detection with nonbleaching multicolor optical immunolabels. Proc Natl Acad Sci 97:996–1001
Sivaraj R, Rahman PK, Rajiv P, Salam HA, Venckatesh R (2014) Biogenic copper oxide nanoparticles synthesis using Tabernaemontana divaricate leaf extract and its antibacterial activity against urinary tract pathogen. Spectrochim Acta Part A 133:178–181
Tamuly C, Saikia I, Hazarika M, Das MR (2014) Reduction of aromatic nitro compounds catalyzed by biogenic CuO nanoparticles. RSC Adv 4:53229–53236
Wang H-K, Yi C-Y, Tian L, Wang W-J, Fang J, Zhao J-H, Shen W-G (2012) Ag-Cu bimetallic nanoparticles prepared by microemulsion method as catalyst for epoxidation of styrene. J Nanomater 2012:453915
Wang L, Tang K, Zhang M, Zhang X, Xu J (2014) Facile synthesis of CuO nanoparticles as anode for lithium ion batteries with enhanced performance. Funct Mater Lett 7:1440008
Wikler MA (2015) Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically; Approved standard. Tenth edition, Clinical and Laboratory Standards Institute, USA
Wongpisutpaisan N, Charoonsuk P, Vittayakorn N, Pecharapa W (2011) Sonochemical synthesis and characterization of copper oxide nanoparticles. Energy Procedia 9:404–409
Yermakov AY, Uimin M, Korolyov A, Mikhalev K, Pirogov A, Teplykh A, Shchegoleva N, Gaviko V, Byzov I, Maikov V (2015) Heterogeneous magnetic state in nanocrystalline cupric oxide CuO. Phys Solid State 57:296–308
Zhang X, Yan S, Tyagi R, Surampalli R (2011) Synthesis of nanoparticles by microorganisms and their application in enhancing microbiological reaction rates. Chemosphere 82:489–494
Zhang J, Ma J, Zhang S, Wang W, Chen Z (2015) A highly sensitive nonenzymatic glucose sensor based on CuO nanoparticles decorated carbon spheres. Sens Actuators B 211:385–391
Zijlstra P, Orrit M (2011) Single metal nanoparticles: optical detection, spectroscopy and applications. Rep Prog Phys 74:106401
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Authors gratefully acknowledge the financial support provided for this work by the Shahid Bahonar University of Kerman.
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Alishah, H., Pourseyedi, S., Ebrahimipour, S.Y. et al. Green synthesis of starch-mediated CuO nanoparticles: preparation, characterization, antimicrobial activities and in vitro MTT assay against MCF-7 cell line. Rend. Fis. Acc. Lincei 28, 65–71 (2017). https://doi.org/10.1007/s12210-016-0574-y
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DOI: https://doi.org/10.1007/s12210-016-0574-y