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Age and gender differences in the influences of eNOS T-786C polymorphism on arteriosclerotic parameters in general population in Japan

  • Regular Article
  • Published:
Environmental Health and Preventive Medicine Aims and scope

Abstract

Objective

The influence of T-786C polymorphism in the promoter region of endothelial nitric oxide synthase (eNOS) on arteriosclerotic parameters by age and gender were examined.

Methods

Brachial-ankle pulse wave velocity (baPWV), heart-rate adjusted augmentation index (AIx@75), pulse pressure (PP) and albumin–creatinine ratio (ACR) were assessed as arteriosclerotic parameters in addition to non-high-density lipoprotein cholesterol (non-HDL-C) to HDL-C (non-HDL-C/HDL-C) ratio in 1499 participants. T-786C polymorphism (rs2070744) was screened using a TaqMan allelic discrimination assay. Analyses of covariance were carried.

Results

Women with the non-C allele showed significantly lower AIx@75 in participants aged <65 years and baPWV in participants aged ≥65 years than those with C allele. In contrast, men with the non-C allele showed significantly higher PP in participants aged <65 years, and higher ACR and non-HDL-C/HDL-C ratio in participants aged ≥65 years. In men on cholesterol-lowering medication, the non-C allele carriers showed significantly higher non-HDL-C compared to those in the C allele carriers.

Conclusions

eNOS T-786C polymorphism is significantly associated with arteriosclerotic parameters accompanied with age and gender differences, possibly involving antioxidative and/or endothelial signaling other than inflammatory signaling.

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Acknowledgments

This work was supported by the Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology, Japan (25460811 and 25350894). We are grateful to Dr. Mitsuru Shiba, Dr. Yuji Uematsu and Ms. Momoko Buyo of Wakayama Medical University for their supports in the examination of participants. We are also grateful to Ms. Mami Murao and Mr. Yushiro Kagami for their participation in the genotyping. We also wish to thank Ms. Toshiko Hanamitsu, a public health nurse, and other members of the Public office on-site in for their assistances in locating and scheduling participants for examinations. We also thank Ms. Yuko Kuwabara for her assistance with data reduction and administration.

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Authors and Affiliations

  1. Department of Public Health, Wakayama Medical University School of Medicine, 811-1 Kimiidera, Wakayama City, Wakayama, 641-8509, Japan

    Marowa Hashimoto & Tatsuya Takeshita

  2. Wakayama Medical University School of Health and Nursing Science, 580 Mikazura, Wakayama City, Wakayama, 641-0011, Japan

    Nobuyuki Miyai, Sonomi Hattori, Akihiko Iwahara, Miyoko Utsumi & Mikio Arita

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  1. Marowa Hashimoto
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Correspondence to Tatsuya Takeshita.

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Hashimoto, M., Miyai, N., Hattori, S. et al. Age and gender differences in the influences of eNOS T-786C polymorphism on arteriosclerotic parameters in general population in Japan. Environ Health Prev Med 21, 274–282 (2016). https://doi.org/10.1007/s12199-016-0527-1

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  • DOI: https://doi.org/10.1007/s12199-016-0527-1

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