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Effect of Pseudopod Extensions on Neutrophil Hemodynamic Transport Near a Wall

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Abstract

During inflammation, circulating neutrophils roll on, and eventually tether to, the endothelial lining of blood vessels, allowing them to exit the bloodstream and enter the surrounding tissue to target pathogens. This process is mediated by the selectin family of adhesion proteins expressed by endothelial cells. Interestingly, only 10% of activated, migrating neutrophils transmigrate into the extravascular space; the other 90% detach from the wall and rejoin the blood flow. Neutrophils extrude pseudopods during the adhesion cascade; however, the transport behavior of this unique cell geometry has not been previously addressed. In this study, a three-dimensional computational model was applied to neutrophils with pseudopodial extensions to study the effect of cell shape on the hydrodynamic transport of neutrophils. The collision time, contact area, contact force, and collision frequency were analyzed as a function of pseudopod length. It was found that neutrophils experience more frequent collisions compared to prolate spheroids of equal volume and length. Longer pseudopods and lower shear rates increase the collision time integral contact area, a predictor of binding potential. Our results indicate that contact between the neutrophil and the vessel wall is found to be focused predominantly on the pseudopod tip.

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Acknowledgment

This work was funded by NIH Grant HL018208, to M.R.K.

Conflicts of interest

Anne Rocheleau, Weiwei Wang, and Michael King have no conflicts of interest to report.

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No human studies were carried out by the authors for this article. No animal studies were carried out by the authors for this article.

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Authors and Affiliations

  1. Meinig School of Biomedical Engineering, Cornell University, 205 Weill Hall, Ithaca, NY, 14853, USA

    Anne D. Rocheleau, Weiwei Wang & Michael R. King

Authors
  1. Anne D. Rocheleau
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  2. Weiwei Wang
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  3. Michael R. King
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Correspondence to Michael R. King.

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Associate Editor Jin-Yu Shao oversaw the review of this article.

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Rocheleau, A.D., Wang, W. & King, M.R. Effect of Pseudopod Extensions on Neutrophil Hemodynamic Transport Near a Wall. Cel. Mol. Bioeng. 9, 85–95 (2016). https://doi.org/10.1007/s12195-015-0420-9

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  • DOI: https://doi.org/10.1007/s12195-015-0420-9

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