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A typical RNA-binding protein gene (AccRBM11) in Apis cerana cerana: characterization of AccRBM11 and its possible involvement in development and stress responses

  • Original Paper
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Cell Stress and Chaperones Aims and scope

Abstract

RNA-binding motif proteins (RBMs) belong to RNA-binding proteins that display extraordinary posttranscriptional gene regulation roles in various cellular processes, including development, growth, and stress responses. Nevertheless, only a few examples of the roles of RBMs are known in insects, particularly in Apis cerana cerana. In the present study, we characterized the novel RNA-binding motif protein 11 from Apis cerana cerana, which was named AccRBM11 and whose promoter sequence included abundant potential transcription factor binding sites that are connected to responses to adverse stress and early development. Quantitative PCR results suggested that AccRBM11 was expressed at highest levels in 1-day postemergence worker bees. AccRBM11 mRNA and protein levels were higher in the poison gland and the epidermis than in other tissues. Moreover, levels of AccRBM11 transcription were upregulated upon all the simulation of abiotic stresses. Furthermore, Western blot analysis indicated that AccRBM11 protein expression levels could be induced under some abiotic stressors, a result that did not completely in agree with the qRT-PCR results. It is also noteworthy that the expression of some genes that connected with development or stress responses were remarkably suppressed when AccRBM11 was silenced, which suggested that AccRBM11 might play a similar role in development or stress reactions with the above genes. Taken together, the data presented here provide evidence that AccRBM11 is potentially involved in the regulation of development and some abiotic stress responses. We expect that this study will promote future research on the function of RNA-binding proteins.

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Abbreviations

5′ UTR:

5′ Untranslated region

A1:

One-day postemergence worker bee

A19:

Nineteen-day postemergence worker bee

A30:

Thirty-day postemergence worker bee

AccRBM11:

Apis cerana cerana RNA-binding motif protein 11

An:

Antennae

CK:

Control check

Dr:

Drone

dsRNA:

Double-stranded RNA

E. coli :

Escherichia coli

E:

Egg

Ep:

Epidermis

GFP:

Green fluorescent protein gene

H2O2 :

Hydrogen peroxide

He:

Hemolymph

HSF:

Heat shock factor

L1:

One-day larval instar

Pr:

Pre-pupal

Mi:

Midgut

Mu:

Muscle

ORF:

Open reading frame

Pb:

Brown-eyed pupae

Pd:

Dark-eyed pupae

Pg:

Poison gland

Pp:

Pink-eyed pupae

Pw:

White-eyed pupae

Qe:

The egg-producing queen bee

qRT-PCR:

Fluorescent real-time quantitative PCR

Qw:

The non-egg-laying queen bee

RBM4:

RNA-binding motif protein 4

RBM7:

RNA-binding motif protein 7

RBMs:

RNA-binding motif proteins

RBPs:

RNA-binding proteins

Re:

Rectum

RRM-RBM7-like:

RNA recognition motif in the RNA-binding protein 7 and similar proteins

RRMs:

RNA-recognizing domains

RNAi:

RNA interference

TFBs:

Transcription factor binding sites

Wi:

Wing

Hs:

Honey sac

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Acknowledgments

This work was financially supported by the earmarked fund for the China Agriculture Research System (No. CARS-45), the National Natural Science Foundation of China (No. 31172275), and Shandong Province Fine Varieties Breeding Projects (2014-2016).

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Authors and Affiliations

  1. State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong, 271018, People’s Republic of China

    Guilin Li, Haihong Jia, Yan Yan, Xingqi Guo & Qinghua Sun

  2. College of Animal Science and Technology, Shandong Agricultural University, Taian, Shandong, 271018, People’s Republic of China

    Hongfang Wang & Baohua Xu

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  1. Guilin Li
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  2. Haihong Jia
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  6. Qinghua Sun
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  7. Baohua Xu
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Correspondence to Qinghua Sun or Baohua Xu.

Electronic supplementary material

Supplementary Fig. 1

The specific detection of anti-AccRBM11. Total protein of overexpression AccRBM11, induced overexpression of pET-30a(+)-AccRBM11 in Transetta cells were loaded in different lanes and the targeted protein was identified using an anti-AccRBM11 (GIF 3 kb)

High resolution image (TIFF 289 kb)

Supplementary Fig. 2

Western blot analysis of AccRBM11. Western blot analysis of AccRBM11 changes after acaricide (a), paraquat (b), cyhalothrin (c), or UV (d) treatments. All of the lanes were loaded with an equivalent concentration of extracted protein from the same treatment at different times. Tubulin was selected as an internal control (GIF 31 kb)

High resolution image (TIFF 717 kb)

Supplementary Table 1

Abiotic stress conditions for each experimental group (DOC 32 kb)

Supplementary Table 2

Primer sequences used in this study (DOC 82 kb)

Supplementary Table 3

Procedures used in this paper (DOC 28 kb)

Supplementary Table 4

Efficiency, coefficient, and number of melting curve peaks for each primer pair used for qRT-PCR (DOC 34 kb)

Supplementary Table 5

Characterization of the genes used in this paper (DOC 59 kb)

Supplementary Table 6

Size of the introns and exons and the A + T content in AccRBM11, AmRBM11-like, AfRBP11, MrRBM11 and BmRBP7 are shown (DOC 44 kb)

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Li, G., Jia, H., Wang, H. et al. A typical RNA-binding protein gene (AccRBM11) in Apis cerana cerana: characterization of AccRBM11 and its possible involvement in development and stress responses. Cell Stress and Chaperones 21, 1005–1019 (2016). https://doi.org/10.1007/s12192-016-0725-1

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