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In vivo behavior of NTBI revealed by automated quantification system

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Abstract

Non-Tf-bound iron (NTBI), which appears in serum in iron overload, is thought to contribute to organ damage; the monitoring of serum NTBI levels may therefore be clinically useful in iron-overloaded patients. However, NTBI quantification methods remain complex, limiting their use in clinical practice. To overcome the technical difficulties often encountered, we recently developed a novel automated NTBI quantification system capable of measuring large numbers of samples. In the present study, we investigated the in vivo behavior of NTBI in human and animal serum using this newly established automated system. Average NTBI in healthy volunteers was 0.44 ± 0.076 μM (median 0.45 μM, range 0.28–0.66 μM), with no significant difference between sexes. Additionally, serum NTBI rapidly increased after iron loading, followed by a sudden disappearance. NTBI levels also decreased in inflammation. The results indicate that NTBI is a unique marker of iron metabolism, unlike other markers of iron metabolism, such as serum ferritin. Our new automated NTBI quantification method may help to reveal the clinical significance of NTBI and contribute to our understanding of iron overload.

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Authors and Affiliations

  1. Department of Oncology for Local Community Cooperation, Asahikawa Medical University Hospital, Asahikawa, Japan

    Satoshi Ito

  2. Division of Gastroenterology and Hematology/Oncology, Department of Medicine, Asahikawa Medical University, 2-1-1-1 Midorigaoka-Higashi, Asahikawa, Hokkaido, 078-8510, Japan

    Satoshi Ito, Katsuya Ikuta, Addo Lynda, Kotoe Shibusa, Yasumichi Toki, Mayumi Hatayama, Masayo Yamamoto, Motohiro Shindo, Yutaka Kohgo & Mikihiro Fujiya

  3. Research and Development Department, Shino-Test Corporation, Sagamihara, Kanagawa, Japan

    Daisuke Kato & Naomi Iizuka

  4. Department of Medical Laboratory and Blood Center, Asahikawa Medical University Hospital, Asahikawa, Hokkaido, Japan

    Noriyasu Niizeki

  5. Department of Gastrointestinal Immunology and Regenerative Medicine, Asahikawa Medical University, 2-1-1-1, Midorigaoka-Higashi, Asahikawa, Hokkaido, 078-8510, Japan

    Yutaka Kohgo

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  1. Satoshi Ito
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  2. Katsuya Ikuta
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  3. Daisuke Kato
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  8. Mayumi Hatayama
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  10. Motohiro Shindo
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  11. Naomi Iizuka
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  13. Mikihiro Fujiya
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Corresponding author

Correspondence to Katsuya Ikuta.

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Conflict of interest

Satoshi Ito, Katsuya Ikuta, Lynda Addo, Kotoe Shibusa, Yasumichi Toki, Mayumi Hatayama, Masayo Yamamoto, Motohiro Shindo, Yutaka Kohgo, Mikihiro Fujiya (Division of Gastroenterology and Hematology/Oncology, Department of Medicine, Asahikawa Medical University and Department of Gastrointestinal Immunology and Regenerative Medicine, Asahikawa Medical University) received research funding from Shino-Test Corporation; this study was also performed in collaboration with Shino-Test Corporation. The above-mentioned departments also received collaborative research funding from Novartis Pharma K.K., Asahi Kasei Medical Co. Ltd., and Chugai Pharmaceutical Co. Ltd. for iron metabolism research work.

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Ito, S., Ikuta, K., Kato, D. et al. In vivo behavior of NTBI revealed by automated quantification system. Int J Hematol 104, 175–181 (2016). https://doi.org/10.1007/s12185-016-2002-6

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  • DOI: https://doi.org/10.1007/s12185-016-2002-6

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