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Evaluation of Freshness of Fishes Using MWCNT/TiO2 Nanobiocomposites Based Biosensor

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Abstract

In present work, we have developed a highly sensitive biosensor to evaluate the freshness of fishes by determining their xanthine level. This is based on xanthine oxidase (XOx) immobilized on the nanocomposite platform of titanium dioxide nanoparticles and multi-walled carbon nanotubes (TiO2/MWCNT). The biosensor exhibited excellent performances like linear relation in the range from 0.5 to 500 μM, detection limit of 0.5 μM (S/N = 3), and a rapid response time of 30 s. The biosensor showed excellent reproducibility, high selectivity, and long-term stability (60 days). The techniques like dynamic light scattering (DLS), scanning electron microscopy (SEM), electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV), and X-ray diffraction (XRD) were used to characterize the nanocomposite modified electrode. The current biosensor is very reliable and stable for the determination of the freshness of fishes.

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Acknowledgments

The present work was supported by SERB, Department of Science and Technology (DST), India, to Dr. Jagriti Narang. Thanks to all scientists referenced throughout the paper whose valuable work has guided the way through to this research work.

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Authors and Affiliations

  1. Amity Institute of Nanotechnology, Amity University, J2 Block, 4th Floor, Noida, (UP), India

    Jagriti Narang & Chaitali Singhal

  2. Holy Family Hospital, Okhla, New Delhi, India

    Nitesh Malhotra

  3. Department of Biochemistry, Maharishi Dayanand University, Rohtak, Haryana, India

    C.S. Pundir

Authors
  1. Jagriti Narang
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  2. Nitesh Malhotra
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  3. Chaitali Singhal
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  4. C.S. Pundir
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Corresponding author

Correspondence to Jagriti Narang.

Ethics declarations

This study was funded by SERB, Department of Science and Technology (DST), India, to Dr. Jagriti Narang.

Conflict of Interest

Jagriti Narang declares that she has no conflict of interest. Nitesh Malhotra declares that he has no conflict of interest. Chaitali Singhal declares that she has no conflict of interest. C.S. Pundir declares that he has no conflict of interest.

Ethical Approval

All applicable international, national, and/or institutional guidelines for the care and use of animals (fish) were followed. This article does not contain any studies with human participants performed by any one of the authors.

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Narang, J., Malhotra, N., Singhal, C. et al. Evaluation of Freshness of Fishes Using MWCNT/TiO2 Nanobiocomposites Based Biosensor. Food Anal. Methods 10, 522–528 (2017). https://doi.org/10.1007/s12161-016-0594-3

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  • DOI: https://doi.org/10.1007/s12161-016-0594-3

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