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Fluorescence Polarization Immunoassay for Highly Efficient Detection of Imidaclothiz in Agricultural Samples

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Abstract

A homogeneous fluorescence polarization immunoassay (FPIA) based on a monoclonal antibody for the detection of imidaclothiz was developed. Two fluorescein-labeled imidaclothiz tracers containing two different bridge lengths were synthesized and purified. Under optimal conditions, the 4-aminofluorescein-labeled imidaclothiz conjugate (AMF-labeled imidaclothiz), which contains a shorter bridge length, showed a higher sensitivity in the FPIA for detecting imidaclothiz, and the full analysis was achieved in less than 11 min. The IC50 and limit of detection (LOD, IC10) were 87.94 ± 10.18 and 0.57 ± 0.16 μg/L, respectively. The spiked recoveries were 83 to 117 % measured in tomato, pear, rice, apple, cucumber, cabbage, and paddy water, with RSDs of 5 to 12 %. Furthermore, the results of FPIA for the authentic samples correlated well with those acquired by HPLC. Overall, the developed FPIA provided a simple, rapid, sensitive, and accurate method that was used for the quantitative detection of imidaclothiz in agricultural samples.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (31301690), the Doctoral Program of Higher Education Research Fund (20130097120006), and the Youth Innovation Fund in Science and Technology of Nanjing Agricultural University (KJ2013008).

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Authors and Affiliations

  1. College of Plant Protection, Nanjing Agricultural University, Nanjing, 210095, China

    Ming Ma, Mo Chen, Lu Feng, Hongjie You, Rui Yang, Xiude Hua & Minghua Wang

  2. State and Local Joint Engineering Research Center of Green Pesticide Invention and Application, Nanjing, 210095, China

    Ming Ma, Mo Chen, Lu Feng, Hongjie You, Rui Yang, Xiude Hua & Minghua Wang

  3. Faculty of Chemistry, M. V. Lomonosov Moscow State University, Moscow, 119991, Russia

    Anna Boroduleva & Sergei A. Eremin

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  1. Ming Ma
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  2. Mo Chen
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  3. Lu Feng
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  4. Hongjie You
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  5. Rui Yang
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  6. Anna Boroduleva
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  7. Xiude Hua
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  8. Sergei A. Eremin
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  9. Minghua Wang
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Correspondence to Minghua Wang.

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Conflict of Interest

Ming Ma declares that he has no conflict of interest. Mo Chen declares that she has no conflict of interest. Lu Feng declares that she has no conflict of interest. Hongjie You declares that he has no conflict of interest. Rui Yang declares that she has no conflict of interest. Anna Boroduleva declares that she has no conflict of interest. Xiude Hua declares that he has no conflict of interest. Sergei A. Eremin declares that he has no conflict of interest. Minghua Wang declares that he has no conflict of interest.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Ma, M., Chen, M., Feng, L. et al. Fluorescence Polarization Immunoassay for Highly Efficient Detection of Imidaclothiz in Agricultural Samples. Food Anal. Methods 9, 2471–2478 (2016). https://doi.org/10.1007/s12161-016-0434-5

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  • DOI: https://doi.org/10.1007/s12161-016-0434-5

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