Abstract
The red yeast rice formulated products, used worldwide as cholesterol-lowering dietary supplements, have the main active ingredient monacolin K which is actually lovastatin registered as the first statin drug. In this work, a rapid and simple square-wave voltammetric method was developed for determination of monacolin K based on the electrochemical reduction of the carbonyl group in lactone ring. Considering the pH dependence of current response and the interconversion of monacolin K to its acid form, the Britton-Robinson buffer of pH 5.0 was selected as a supporting electrolyte for analytical measurements. The net square-wave voltammetric response of monacolin K was shifted towards more negative potential values by increasing the concentration of analytes. The peak current was linearly proportional to monacolin K concentration in the range of 0.25–20 μg/mL. The fully validated method was successfully applied for fast screening and direct voltammetric determination of monacolin K in red yeast rice products formulated in various dosage forms. To evaluate the proposed voltammetric method, the results obtained were compared with those obtained by HPLC methods.
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Conflict of Interest
Biljana Nigović declares that she has no conflict of interest. Mario Završki declares that he has no conflict of interest. Miranda Sertić declares that she has no conflict of interest. Ana Mornar declares that she has no conflict of interest. This article does not contain any studies with human or animal subjects.
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Nigović, B., Završki, M., Sertić, M. et al. Simple and Fast Voltammetric Method for Assaying Monacolin K in Red Yeast Rice Formulated Products. Food Anal. Methods 8, 180–188 (2015). https://doi.org/10.1007/s12161-014-9890-y
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DOI: https://doi.org/10.1007/s12161-014-9890-y