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Effect of Temperature and Light Exposure on the Detection of Total Intact Glucosinolate Content by LC-ESI-MS in Broccoli Leaves

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Abstract

Total intact glucosinolate content in broccoli leaf extracts (Ramoso calabrese cultivar) has been determined by liquid chromatography coupled to tandem mass spectrometry with the aim of detecting potential differences in this value due to the effects of the drying temperature or the working and storage conditions (temperature and light exposure). Those broccoli leaf extracts were obtained with two different sample treatments based on heating the sample (microwave or oven), and using boiling water as extraction solvent. Significant differences were observed in the total intact glucosinolate content depending on the drying temperature and light exposure under typical working conditions. Meanwhile, those differences were less remarkable when the extracts were stored at low temperature and protected from light exposure.

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Abbreviations

ALY:

Glucoalyssin

DW:

Dry weight

ESI:

Electrospray ionization

BC:

Glucobrassicin

GER:

Glucoerucin

GIB:

Glucoiberin

GLSs:

Glucosinolates

GRA:

Glucoraphanin

GST:

Gluconasturtiin

4ME:

4-Methoxyglucobrassicin

MW:

Microwave

NEO:

Neoglucobrassicin

4OH:

4-Hydroxyglucobrassicin

SIN:

Sinigrin

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Acknowledgements

A.M. Ares thanks the Spanish Ministry "Educación, Cultura y Deporte" for her Ph.D grant. This work was funded by the Spanish Ministry "Economía y Competitividad" and INIA (Project RTA2012-00076-C02-02).

Compliance with Ethics Requirements

This article does not contain any studies with human or animal subjects.

Conflict of Interest

Ana M. Ares declares that she has no conflict of interest. María J. Nozal declares that she has no conflict of interest. José L. Bernal declares that he has no conflict of interest. José Bernal declares that he has no conflict of interest.

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Authors and Affiliations

  1. IU CINQUIMA, Analytical Chemistry Group, University of Valladolid, 47011, Valladolid, Spain

    Ana M. Ares, María J. Nozal, José L. Bernal & José Bernal

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  1. Ana M. Ares
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  2. María J. Nozal
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  3. José L. Bernal
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  4. José Bernal
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Correspondence to José Bernal.

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Ares, A.M., Nozal, M.J., Bernal, J.L. et al. Effect of Temperature and Light Exposure on the Detection of Total Intact Glucosinolate Content by LC-ESI-MS in Broccoli Leaves. Food Anal. Methods 7, 1687–1692 (2014). https://doi.org/10.1007/s12161-014-9806-x

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  • DOI: https://doi.org/10.1007/s12161-014-9806-x

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