Skip to main content

Advertisement

SpringerLink
Log in

A Rapid UPLC-PAD Fingerprint Analysis of Chrysanthemum morifolium Ramat Combined with Chemometrics Methods

  • Published:
Food Analytical Methods Aims and scope Submit manuscript

Abstract

A novel approach for fingerprint analysis of Chrysanthemum morifolium Ramat was developed by combining chemometrics methods such as similarity analysis, hierarchical cluster analysis, and principal component analysis with ultra-performance liquid chromatography. The chromatographic separation was accomplished by gradient elution with mobile phase consisting of acetonitrile and phosphate buffer in 10 min prior to conventional liquid chromatography which usually takes over 1 h. Good reproducibility, precision, and accuracy were obtained with relative standard deviations below 2.10 % of six typical components. Consistent results were obtained in accordance with their cultivated sources. The proposed method above was confirmed to be more rapid and suitable, thus providing important criteria for further quality control of C. morifolium Ramat.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  • Chen T, Li LP, Lu XY, Jiang HD, Zeng S (2007) Absorption and excretion of luteolin and apigenin in rats after oral administration of Chrysanthemum morifolium extract. J Agric Food Chem 55:273–277

    Article  CAS  Google Scholar 

  • Cheng HY, Feng QY, Cao YH, Gong LD, Hou JX, Wang Y (2007) Determination of active ingredients in Chrysanthemum and development of fingerprints by high performance liquid chromatography. J Anal Sci 23(3):257–262

    Article  CAS  Google Scholar 

  • Clifford MN, Wu WG, Kirkpatrick J, Kuhnert N (2007) Profiling the chlorogenic acids and other caffeic acid derivatives of herbal chrysanthemum by LC-MSn. J Agric Food Chem 55:929–936

    Article  CAS  Google Scholar 

  • Critchfield JW, Butera ST, Folks TM (1996) Inhibition of HIV activation in latently infected cells by flavonoid compounds. AIDS Res Hum Retrovir 12(1):39–46

    Article  CAS  Google Scholar 

  • Jiang HD, Xia Q, Xu WH, Zheng M (2004) Chrysanthemum morifolium attenuated the reduction of contraction of isolated rat heart and cardiomyocytes induced by ischemia/reperfusion. Pharmazie 59:565–567

    CAS  Google Scholar 

  • Jiang HD, Hu BB, Zeng S (2005a) Fingerprint analysis for the quality assessment of Flos Chrysanthemi. Chin Pharm J 40(8):578–581

    CAS  Google Scholar 

  • Jiang HD, Wang LF, Zhou XM, Xia Q (2005b) Vasorelaxant effect and underlying mechanism of EtOAc extract from Chrysanthemum morifolium in rat thoracic aorta. Chin J Pathophysiol 21:334–338

    CAS  Google Scholar 

  • Jin UH, Lee JY, Kang SK, Kim JK, Park WH, Kim JG, Moon SK, Kim CH (2005) A phenolic compound, 5-caffeoylquinic acid (chlorogenic acid), is a new type and strong matrix metalloproteinase-9 inhibitor: isolation and identification from methanol extract of Euonymus alatus. Life Sci 77:2760–2769

    Article  CAS  Google Scholar 

  • King PJ, Ma GX, Miao WF, Jia Q, McDougall BR, Reinecke MG, Cornell C, Kuan J, Kim TR, Robinson WE, Jr J (1999) Structure activity relationships: analogues of the dicaffeoylquinic and dicaffeoyltartaric acids as potent inhibitors of human immunodeficiency virus type 1 integrase and replication. Med Chem 42:497–509

    Article  CAS  Google Scholar 

  • Kong WJ, Wang JB, Zang QC, Xing XY, Zhao YL, Liu W, Jin C, Li ZL, Xiao XH (2011) Fingerprint-efficacy study of artificial Calculusbovis in quality control of Chinese materia medica. Food Chem 127:1342–1347

    Article  CAS  Google Scholar 

  • Lai JP, Lim YH, Su J, Shen HM, Ong CN (2007) Identification and characterization of major flavonoids and caffeoylquinic acids in three Compositae plants by LC/DAD-APCI/MS. J Chromatogr B 848:215–225

    Article  CAS  Google Scholar 

  • Lee JS, Kim HJ, Lee YS (2003) A new anti-HIV flavonoid from glucoronide from Chrysanthemum marifolium. Planta Med 69:859–861

    Article  CAS  Google Scholar 

  • Li SL, Song JZ, Qiao CF, Zhou Y, Xu HX (2010) UPLC-PDA-TOFMS based chemical profiling approach to rapidlly evaluate chemical consistency between traditional and dispensing granule decoctions of traditional medicine combinatorial formulae. J Pharm Biomed 52:468–478

    Article  CAS  Google Scholar 

  • Li JH, Liu X, Han S, Li J, Xu Q, Xu H, Wang YW, Liu F, Zhang ZH (2012) Analysis of ochratoxin A in wine by high-resolution UHPLC-MS. Food Anal Methods 5:1506–1513

    Article  Google Scholar 

  • Lin LZ, Harnly JM (2010) Identification of the phenolic components of chrysanthemum flower (Chrysanthemum morifolium Ramat). Food Chem 120:319–326

    Article  CAS  Google Scholar 

  • Lu HM, Liang YZ, Chen S (2006) Identification and quality assessment of Houttuynia cordata injection using GC-MS fingerprint: a standardization approach. J Ethnopharmacol 105(3):436–440

    Article  CAS  Google Scholar 

  • Nguyen DTT, Guillarme D, Rudaz S, Veuthey JL (2006) Chromatographic behaviour and comparison of column packed with sub-2 μm stationary phases in liquid chromatography. J Chromatogr A 1128:105–113

    Article  CAS  Google Scholar 

  • Pharmacopoeia of People’s Republic of China (2010) The State of Pharmacopoeia Commission of People’s Republic of China, vol I. Chemical Industry Press, Beijing, p 292

    Google Scholar 

  • Selected Words of Chinese Bencao (1999) State administration of traditional Chinese medicine, 7th edn. Scientific and Technical Publishers, Shanghai, p 805

    Google Scholar 

  • Shen Z, Zhang WT, Hua YF, Zhao WL (2010) Fingerprinting analysis of four variants of Chrysanthemi Morifoli Flos by RP-HPLC. Chin Herb Med 2(2):153–156

    CAS  Google Scholar 

  • Shi RX, Ong CN, Shen HM (2005) Protein kinase C inhibition and X-linked inhibitor of apoptosis protein degradation contribute to the sensitization effect of luteolin on tumor necrosis factor-related apoptosis-inducing ligand-induced apoptosis in cancer cells. Cancer Res 65:7815–7823

    Article  CAS  Google Scholar 

  • Singh RP, Agrawal P, Yim DS, Agarwal C, Agarwal R (2005) Acacetin inhibits cell growth and cell cycle progression, and induces apoptosis in human prostate cancer cells: structure–activity relationship with linarin and linarin acetate. Carcinogenesis 26:845–854

    Article  CAS  Google Scholar 

  • Sun JH, Chen P (2012) Chromatographic fingerprint analysis of yohimbe bark and related dietary supplements using UHPLC/UV/MS. J Pharm Biomed 61:142–149

    Article  CAS  Google Scholar 

  • Tang YY, Lu HF, Lin HY, Shin YC, Hwang DF (2012) Development of a quantitative multi-class method for 18 antibiotics in chicken, pig, and fish muscle using UPLC-MS/MS. Food Anal Methods 5:1459–1468

    Article  Google Scholar 

  • Tsai FJ, Lin CW, Lai CC, Lan YC, Lai CH, Hung CH, Hsueh KC, Lin TH, Chang HC, Wan L, Sheu JJC, Lin YJ (2011) Kaempferol inhibits enterovirus 71 replication and internal ribosome entry site (IRES) activity through FUBP and HNRP proteins. Food Chem 128:312–322

    Article  CAS  Google Scholar 

  • Xu CJ, Liang YZ, Chau FT, Heyden Y (2006) Pretreatments of chromatographic fingerprints for quality control of herbal medicines. J Chromatogr A 1134:253–259

    Article  CAS  Google Scholar 

Download references

Acknowledgments

We thank the National Natural Science Foundation of China [21206148] for financial support.

Conflict of Interest

Xianrui Liang declares that she has no conflict of interest. Hong Wu declares that she has no conflict of interest. Weike Su declares that he has no conflict of interest. This article does not contain any studies with human or animal subjects.

Author information

Authors and Affiliations

  1. Key Laboratory for Green Pharmaceutical Technologies and Related Equipment of Ministry of Education, College of Pharmaceutical Sciences, Zhejiang University of Technology, Hangzhou, 310014, China

    Xianrui Liang, Hong Wu & Weike Su

Authors
  1. Xianrui Liang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Hong Wu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Weike Su
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Weike Su.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Liang, X., Wu, H. & Su, W. A Rapid UPLC-PAD Fingerprint Analysis of Chrysanthemum morifolium Ramat Combined with Chemometrics Methods. Food Anal. Methods 7, 197–204 (2014). https://doi.org/10.1007/s12161-013-9618-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12161-013-9618-4

Keywords

Search

Navigation