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Analysis of Water-Soluble Vitamins in Honey by Isocratic RP-HPLC

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Abstract

An isocratic RP-HPLC method has been developed for the identification and quantification of water-soluble vitamins in honey. The mobile phase tested was an aqueous solution of sulphuric acid and the detection was carried out simultaneously by UV and fluorescence. The separation of vitamins C (l-ascorbic acid), B1 (thiamine), B3N (nicotinamide), B3H (nicotinic acid), B5 (d-pantothenic acid) and B6 (pyridoxine) is achieved in these conditions in 15 min. The addition to the mobile phase of methanol 2 % v/v reduced significantly the analysis time in the separation of these vitamins up to 10 min. Moreover, in presence of a cationic surfactant hexadecyltrimethylammonium bromide (CTAB) in the mobile phase, the separation of vitamin C, B1, B3N, B3H, B2 (riboflavin) and B6 is possible in 6 min. The combination of both mobile phases, H2SO4/methanol and H2SO4/methanol/CTAB, has been applied to the analysis, in isocratic mode, of several monofloral honeys (rosemary, thyme, lavender, chestnut, echium) and a honeydew honey in a short time analysis.

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Authors and Affiliations

  1. Analytical Chemistry Department, Faculty of Chemistry, University of Alcalá, Ctra. Madrid-Barcelona Km. 33,6, 28871, Alcalá de Henares, Madrid, Spain

    Virginia León-Ruiz, Soledad Vera & María Paz San Andrés

  2. Beekeeping Research Centre, C/ Extramuros, s/n, 19180, Marchamalo, Guadalajara, Spain

    Virginia León-Ruiz & Amelia V. González-Porto

Authors
  1. Virginia León-Ruiz
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  2. Soledad Vera
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  3. Amelia V. González-Porto
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  4. María Paz San Andrés
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Correspondence to María Paz San Andrés.

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León-Ruiz, V., Vera, S., González-Porto, A.V. et al. Analysis of Water-Soluble Vitamins in Honey by Isocratic RP-HPLC. Food Anal. Methods 6, 488–496 (2013). https://doi.org/10.1007/s12161-012-9477-4

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  • DOI: https://doi.org/10.1007/s12161-012-9477-4

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