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A Promising Low Beta-Glucan Barley Mutation of m351 for Better Bioethanol Production Use

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Abstract

Bioethanol is an important liquid fuel complement. Barley is a potential alternative raw material for ethanol production because its by-product is a nutritious feed. The barley m351mutant, which has a mutation for low beta-glucan content, was tested for its ethanol production efficiency and feed fraction quality in a pilot fermentation experiment. Grain samples of m351 and the corresponding wild type of Harrington were either treated or untreated with beta-glucanase during the ethanol production process. Compared to the corresponding wild-type line, m351 produced the same amount of ethanol. More importantly, the feed fraction derived from the fermentation process showed 5 % more protein and 7 % less beta-glucan than that from the wild type, indicating that the feed fraction from m351 was more valuable. The experiments provided evidence that m351 is a useful genetic resource for developing barley cultivars for use in bioethanol production.

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References

  1. Sanchez OJ, Cardona CA (2008) Trends in biotechnological production of fuel ethanol from different feedstocks. Biores Technol 99:5270–5295

    Article  CAS  Google Scholar 

  2. Chum HL, Warner E, Seabra JEA, Macedo IC (2014) A comparison of commercial ethanol production systems from Brazilian sugarcane and US corn. Biofuels Bioprod Biorefin 8:205–223

    Article  CAS  Google Scholar 

  3. Lee RA, Lavoie JM (2013) From first- to third-generation biofuels: challenges of producing a commodity from a biomass of increasing complexity. Animal Front 3:6–11

    Article  Google Scholar 

  4. Dale BE, Kim S (2004) Global potential bioethanol production from wasted crops and crop residues. Biomass Bioenergy 26:361–375

    Article  Google Scholar 

  5. Gibreel A, Sandercock JR, Lan J, Goonewardene LA, Zijlstra RT, Curtis JM, Bressler DC (2009) Fermentation of barley by using Saccharomyces cerevisiae: examination of barley as a feedstock for bioethanol production and value-added products. Appl Environ Microbiol 75:1363–1372

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  6. Hicks KB, Flores RA, Taylor F, McAloon AJ, Moreau RA, Johnston D, Senske GE, Brooks WS, Griffey CA (2005) Barley: a potential feedstock for fuel ethanol, p. 25-29. In: U.S. Proceedings of the Fourth International Starch Conference. University of Illinois, Urbana-Champaign, IL

  7. Griffey C, Brooks W, Kurantz M, Thomason W, Taylor F, Obert D, Moreau R, Flores R, Sohn M, Hicks K (2010) Grain composition of Virginia winter barley and implications for use in feed, food, and biofuels production. J Cereal Sci 51:41–49

    Article  CAS  Google Scholar 

  8. Nghiem NP, Hicks KB, Johnston DB, Senske G, Kurantz M, Li M, Shetty J, Konieczny-Janda G (2010) Production of ethanol from winter barley by the EDGE (enhanced dry grind enzymatic) process. Biotechnol Biofuels 3:8

    Article  PubMed Central  PubMed  Google Scholar 

  9. Nghiem NP, Ramírez EC, McAloon AJ, Yee W, Johnston DB, Hicks KB(2011) Economic analysis of fuel ethanol production from winter hulled barley by the EDGE (Enhanced Dry Grind Enzymatic) process. Bioresource Technology 102:6696–6701

  10. Nghiem NP, Taylor F, Hicks KB, Johnston DB, Shetty JK (2011) Scale-up of ethanol production from winter barley by the EDGE (enhanced dry grind enzymatic) process in fermentors up to 300 liters. Appl Biochem Biotechnol 165:870–882

    Article  CAS  PubMed  Google Scholar 

  11. Hu G, Burton C, Zong H, Jackson E (2014) A mutation of the cellulose-synthase-like (CslF6) gene in barley (Hordeum vulgare L.) partially affects the β-glucan content in grains. J Cereal Sci 59:189–195

    Article  CAS  Google Scholar 

  12. Hu G, Burton C (2008) Modification of the standard enzymatic protocol to a cost-efficient format for mixed-linkage (1-3, 1-4)-β-D-glucan measurement. Cereal Chem 85:648–653

    Article  CAS  Google Scholar 

  13. Varga E, Thomsen AB, Feng L(2005) “Ethanol production from corn, corn stover and corn cob from the Jilin province of China.” In: Technologies for sustainable energy development in the long term. Proceedings, Riso International Energy Conference, pp. 369-378

  14. Mielenz JR (2001) Ethanol production from biomass: technology and commercialization status. Curr Opin Microbiol 4:324–329

    Article  CAS  PubMed  Google Scholar 

  15. Wall JS, James C, Cavins JF (1971) Nutritive value of protein in hominy feed fractions. Cereal Chem 48:456

    CAS  Google Scholar 

  16. Wu YV, Sexson KR, Lagoda AA (1985) Protein-rich alcohol fermentation residues from corn dry-milled fractions. Cereal Chem 62:470–473

    CAS  Google Scholar 

Download references

Acknowledgments

The authors would like to thank Dr. Kevin Hicks for the critical reading of the manuscript. The experiment was supported by the biofuel research grant from USDA-ARS.

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Authors and Affiliations

  1. USDA-ARS, 1691 S 2700 W, Aberdeen, ID, 83210, USA

    Gongshe Hu & Sherry R. Ellberg

  2. National Corn-to-Ethanol Research Center, Edwardsville, IL, USA

    Sabrina Trupia

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  1. Gongshe Hu
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  2. Sabrina Trupia
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  3. Sherry R. Ellberg
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Correspondence to Gongshe Hu.

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Hu, G., Trupia, S. & Ellberg, S.R. A Promising Low Beta-Glucan Barley Mutation of m351 for Better Bioethanol Production Use. Bioenerg. Res. 8, 1158–1164 (2015). https://doi.org/10.1007/s12155-014-9569-7

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  • DOI: https://doi.org/10.1007/s12155-014-9569-7

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