Abstract
Efficient trafficking of ubiquitinated receptors (cargo) to endosomes requires the recruitment of adaptor proteins that exhibit ubiquitin-binding domains for recognition and transport. Tom1 is an adaptor protein that not only associates with ubiquitinated cargo but also represents a phosphoinositide effector during specific bacterial infections. This phosphoinositide-binding property is associated with its N-terminal Vps27, Hrs, STAM (VHS) domain. Despite its biological relevance, there are no resonance assignments of Tom1 VHS available that can fully characterize its molecular interactions. Here, we report the nearly complete 1H, 15N, and 13C backbone resonance assignments of the VHS domain of human Tom1.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Ankem G, Mitra S, Sun F, Moreno AC, Chutvirasakul B, Azurmendi HF, Li L, Capelluto DG (2011) The C2 domain of Tollip, a Toll-like receptor signalling regulator, exhibits broad preference for phosphoinositides. Biochem J 435:597–608
Boal F, Mansour R, Gayral M, Saland E, Chicanne G, Xuereb JM, Marcellin M, Burlet-Schiltz O, Sansonetti PJ, Payrastre B et al (2015) TOM1 is a PI5P effector involved in the regulation of endosomal maturation. J Cell Sci 128:815–827
Brissoni B, Agostini L, Kropf M, Martinon F, Swoboda V, Lippens S, Everett H, Aebi N, Janssens S, Meylan E et al (2006) Intracellular trafficking of interleukin-1 receptor I requires Tollip. Curr Biol 16:2265–2270
Campsteijn C, Vietri M, Stenmark H (2016) Novel ESCRT functions in cell biology: spiraling out of control? Curr Opin Cell Biol 41:1–8
Cullen PJ (2008) Endosomal sorting and signalling: an emerging role for sorting nexins. Nat Rev Mol Cell Biol 9:574–582
Delaglio F, Grzesiek S, Vuister GW, Zhu G, Pfeifer J, Bax A (1995) NMRpipe—a multidimensional spectral processing system based on Unix pipes. J Biomol NMR 6:277–293
Goddard TD, Kneller DG (2008) Sparky-NMR assignment and integration software. SPARKY 3, University of California, San Francisco
Grzesiek S, Anglister J, Bax A (1993) Correlation of backbone amide and aliphatic side-chain resonances in C-13/N-15-enriched proteins by isotropic mixing of C-13 magnetization. J Magn Reson 101:114–119
Husnjak K, Dikic I (2012) Ubiquitin-binding proteins: decoders of ubiquitin-mediated cellular functions. Ann Rev Biochem 81:291–322
Johnson BA, Blevins RA (1994) NMR view: a computer program for the visualization and analysis of NMR data. J Biomol NMR 4:603–614
Katoh Y, Shiba Y, Mitsuhashi H, Yanagida Y, Takatsu H, Nakayama K (2004) Tollip and Tom1 form a complex and recruit ubiquitin-conjugated proteins onto early endosomes. J Biol Chem 279:24435–24443
Misra S, Beach BM, Hurley JH (2000) Structure of the VHS domain of human Tom1 (target of myb 1): insights into interactions with proteins and membranes. Biochemistry 39:11282–11290
Mitra S, Traughber CA, Brannon MK, Gomez S, Capelluto DG (2013) Ubiquitin interacts with the Tollip C2 and CUE domains and inhibits binding of Tollip to phosphoinositides. J Biol Chem 288:25780–25791
Muhandiram DR, Kay LE (1994) Gradient-enhanced triple-resonance 3-dimensional NMR experiments with improved sensitivity. J Magn Reson 103:203–216
Ren X, Hurley JH (2010) VHS domains of ESCRT-0 cooperate in high-avidity binding to polyubiquitinated cargo. EMBO J 29:1045–1054
Shen Y, Delaglio F, Cornilescu G, Bax A (2009) TALOS + : a hybrid method for predicting protein backbone torsion angles from NMR chemical shifts. J Biomol NMR 44:213–223
Vranken WF, Boucher W, Stevens TJ, Fogh RH, Pajon A, Llinas M, Ulrich EL, Markley JL, Ionides J, Laue ED (2005) The CCPN data model for NMR spectroscopy: development of a software pipeline. Proteins 59:687–696
Xiao S, Brannon MK, Zhao X, Fread KI, Ellena JF, Bushweller JH, Finkielstein CV, Armstrong GS, Capelluto DG (2015) Tom1 modulates binding of Tollip to phosphatidylinositol 3-phosphate via a coupled folding and binding mechanism. Structure 23:1910–1920
Xiao S, Ellena JF, Armstrong GS, Capelluto DG (2016) Structure of the GAT domain of the endosomal adapter protein Tom1. Data Brief 7:344–348
Yamakami M, Yoshimori T, Yokosawa H (2003) Tom1, a VHS domain-containing protein, interacts with tollip, ubiquitin, and clathrin. J Biol Chem 278:52865–52872
Zhu L, Wang L, Luo X, Zhang Y, Ding Q, Jiang X, Wang X, Pan Y, Chen Y (2012) Tollip, an intracellular trafficking protein, is a novel modulator of the transforming growth factor-beta signaling pathway. J Biol Chem 287:39653–39663
Acknowledgments
We thank Dr. Janet Webster for assistance during preparation of the manuscript. This work was funded by an American Heart Association Grant-in-Aid (13GRNT16960080) to D.G.S.C.
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Conflict of interest
The authors declare that they have no conflict of interest.
Rights and permissions
About this article
Cite this article
Ellena, J.F., Xiong, W., Zhao, X. et al. Backbone 1H, 15N, and 13C resonance assignments of the Tom1 VHS domain. Biomol NMR Assign 11, 1–4 (2017). https://doi.org/10.1007/s12104-016-9709-4
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12104-016-9709-4