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MicroRNA-10b indicates a poor prognosis of non-small cell lung cancer and targets E-cadherin

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Abstract

Background

MicroRNA-10b(miR-10b) has been reported to be dysregulated in some types of cancer and to play an important role in invasion and metastasis. It was previously found to be a tumor enhancer in NSCLC; however, its clinical significance in NSCLC has not been evaluated.

Methods

We compared the expression levels of miR-10b in 73 pairs of NSCLC tissues and the corresponding noncancerous tissues, as well as in human lung cancer cell line A549 and NHBE cell line by qRT-PCR. Expression of E-cadherin (E-cad) was detected using RT-PCR and Western blot analysis. The disease-specific survival (DSS) was analyzed by log-rank test, and survival curves were plotted according to Kaplan–Meier.

Results

MiR-10b was significantly upregulated in NSCLC tissues as well as in A549 cell line. The relative miR-10b expression levels were significantly positively correlated with TNM stage (p = 0.01) and regional lymph node involvement (p < 0.001). Kaplan–Meier analysis showed that patients with higher levels of miR-10b had significantly poorer survival than those with lower expression of this miRNA in patients, with a 5-year DSS of 29.5 and 63.8 %, respectively (p = 0.003). The E-cad mRNA and protein were overexpressed in miR-10b-suppressed cells compared with controls.

Conclusion

Our results indicated that miR-10b expression was an independent prognostic factor in NSCLC patients. Furthermore, miR-10b might be necessary for driving the expression of E-cad in NSCLC.

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Conflict of interest

The authors declare that they have no competing interests.

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Authors and Affiliations

  1. Department of Thoracic Surgery, Weifang People’s Hospital, Weifang, 261041, Shandong, China

    J. Zhang, L. Xu, Z. Yang, H. Lu, D. Hu, W. Li, Z. Zhang & B. Liu

  2. Department of Oncology Surgery, Weifang People’s Hospital, 151 Guang Wen Street, Weifang, 261041, Shandong, China

    S. Ma

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  1. J. Zhang
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  2. L. Xu
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  3. Z. Yang
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  4. H. Lu
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  5. D. Hu
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  6. W. Li
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  7. Z. Zhang
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  8. B. Liu
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  9. S. Ma
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Correspondence to S. Ma.

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Zhang, J., Xu, L., Yang, Z. et al. MicroRNA-10b indicates a poor prognosis of non-small cell lung cancer and targets E-cadherin. Clin Transl Oncol 17, 209–214 (2015). https://doi.org/10.1007/s12094-014-1213-7

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  • DOI: https://doi.org/10.1007/s12094-014-1213-7

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