Abstract
In the first part of this paper, gut microbial difference of two genotypes mice was researched. The gut microbial community of type 2 diabetes animal model KKAy mice and normal C57BL/6J mice had clear distinctions in DGGE (denaturing gradient gel electrophoresis) profiles. The pairwise similarity coefficient (C s ) was only 26–44 % between KKAy and C57BL/6J, but C s was 82–100 % among same genotypes mice. Thirteen dominant bands were cloned from DGGE profiles to exhibit difference on gut microbial structure further. In the second part of this paper, the influence of hypoglycemic drug Pioglitazone on the gut microbes in KKAy mice was researched by gut microbial diversity analysis and principal component analysis (PCA). The results showed that Pioglitazone reduced the gut microbial diversity slightly and changed gut microbial structure of KKAy mice to that of normal C57BL/6J mice.
Similar content being viewed by others
References
Yadav H, Jain S, Sinha PR (2007) Antidiabetic effect of probiotic dahi containing Lactobacillus acidophilus and Lactobacillus casei in high fructose fed rats. Nutrition 23:62–68
Harris K, Kassis A, Major G, Chou CJ (2012) Is the gut microbiota a new factor contributing to obesity and its metabolic disorders? J Obes 2012:1–14
Le Roy T, Llopis M, Lepage P, Bruneau A et al (2012) Intestinal microbiota determines development of non-alcoholic fatty liver disease in mice. Gut 0:1–8
Ventura M, O’Flaherty S, Claesson MJ, Turroni F et al (2009) Genome-scale analyses of health-promoting bacteria: probiogenomics. Nat Rev Microbiol 7:61–71
Qin J, Li R, Rase J, Arumugam M et al (2010) A human gut microbial gene catalogue established by metagenomic sequencing. Nature 464:59–65
Bäckhed F, Ley RE, Sonnenburg JL, Peterson DA, Gordon JI (2005) Host-bacterial mutualism in the human intestine. Science 307:1915–1920
Bäckhed F, Ding H, Wang T, Hooper LV et al (2004) The gut microbiota as an environmental factor that regulates fat storage. Proc Natl Acad Sci USA 101:15718–15723
Wang Y, Devkota S, Musch MW, Jabri B et al (2010) Regional mucosa-associated microbiota determine physiological expression of TLR2 and TLR4 in murine colon. PLoS ONE 5:e13607
Korbie DJ, Mattick JS (2008) Touchdown PCR for increased specificity and sensitivity in PCR amplification. Nat Protoc 3:1452–1456
Thompson JR, Marcelino LA, Polz MF (2002) Heteroduplexes in mixed-template amplifications: formation, consequence and elimination by ‘reconditioning PCR’. Nucleic Acids Res 30:2083–2088
Kusumakar AL, Savita Malik Y, Minakshi Prasad G (2007) Detection of human rotavirus in hospitalized diarrheic children in central India. Indian J Microbiol 47:373–376
Diserud OH, Odegaard F (2007) A multiple-site similarity measure. Biol Lett 3:20–22
Lyautey E, Lacoste B, Ten-Hage L, Rols JL, Garabetian F (2005) Analysis of bacterial diversity in river biofilms using 16S rDNA PCR-DGGE: methodological settings and fingerprints interpretation. Water Res 39:380–388
Sundar SK, Palavesam A, Parthipan B (2011) AM fungal diversity in selected medicinal plants of Kanyakumari District, Tamil Nadu, India. Indian J Microbiol 51:259–265
Spor A, Koren O, Ley R (2011) Unravelling the effects of the environment and host genotype on the gut microbiome. Nat Rev Microbiol 9:279–290
Shi L, Cai Y, Yang H, Xing P, Li P et al (2009) Phylogenetic diversity and specificity of bacteria associated with Microcystis aeruginosa and other cyanobacteria. J Environ Sci 21:1581–1590
Evans JL, Maddux BA, Goldfine ID (2005) The molecular basis for oxidative stress-induced insulin resistance. Antioxid Redox Signal 7:1040–1052
Ganesan S, Raja S, Sampathkumar P et al (2011) Isolation and screening of α-glucosidase enzyme inhibitor producing marine actinobacteria. Afr J Microbiol Res 5:3437–3445
Cani PD, Amar J, Iglesias MA et al (2007) Metabolic endotoxemia initiates obesity and insulin resistance. Diabetes 56:1761–1772
Acknowledgments
This work has been supported by the National Natural Science Foundation of China (30771310) and the Program of Science and Technology of Shanxi province, China (20120311006-1).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Li, Y., Han, L., Xu, M. et al. The Primary Research on the Gut Microbes in KKAy Mice. Indian J Microbiol 54, 12–19 (2014). https://doi.org/10.1007/s12088-013-0410-3
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12088-013-0410-3