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Expression of CagL from Helicobacter pylori and Preliminary Study of its Biological Function

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Abstract

Helicobacter pylori (H. pylori) is a highly successful human-specific gastric pathogen, infecting over half the world’s population. Virulent H. pylori isolates harbour the cytotoxin-associated genes pathogenicity island (cag-PAI), the majority of which have no known function. In this study, we used cell infection assay and reverse transcriptase PCR, identified that CagL recombinant protein, one of the cag-PAI proteins, induced GES-1 cells to express cytokine IL-8. Then we performed western blot and translocation assay. Our result showed CagL polyclonal antibody counteracted translocation of CagA. This will provide a foundation for the further studies on its biological function.

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Acknowledgments

We thank Prof. Seung-chul Baik (Gyeongsang National University College of Medicine, Republic of Korea) for kindly providing us the suicide plasmid pBlue-KM40 and technical assistant for mutant construct of H. pylori. This work was supported by grants from the National Natural Science Funds (81271795) and Research Foundation for Advanced Talents in Jiangsu University (12JDG064).

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Authors and Affiliations

  1. School of Medical Science and Laboratory Medicine, Jiangsu University, 301 Xuefu Road, Zhenjiang, 212013, Jiangsu, People’s Republic of China

    Hua Wang, Shiteng Huang, Jun Han, Xianwei Guan & Shihe Shao

  2. Department of Clinical Hospital, Jiangsu University, Zhenjiang, 212011, Jiangsu, China

    Jianzhong Zhao

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  1. Hua Wang
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  2. Shiteng Huang
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  3. Jianzhong Zhao
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  4. Jun Han
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  5. Xianwei Guan
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  6. Shihe Shao
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Corresponding author

Correspondence to Shihe Shao.

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Hua Wang and Shiteng Huang contributed equally to this work.

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Wang, H., Huang, S., Zhao, J. et al. Expression of CagL from Helicobacter pylori and Preliminary Study of its Biological Function. Indian J Microbiol 53, 36–40 (2013). https://doi.org/10.1007/s12088-012-0341-4

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  • DOI: https://doi.org/10.1007/s12088-012-0341-4

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