Abstract
Helicobacter pylori is a gram-negative bacillus causing benign and malignant gastric diseases such as gastroesophageal reflux (GER). In larynx, H. pylori causes chronic inflammation and mucosal destruction that may lead to malignant changes. Although, H. pylori poses several virulence factors, cagA is probably the main factor in this regard. To evaluate the role of cagA gene in laryngeal squamous cell carcinoma (LSCC), a case–control study was conducted on patients with laryngeal complaints during 2010–2012. Seventy-two patients with LSCC (case group) and 72 patients without malignancy (control group) were included in the study. The H. pylori and cagA factor were assessed in laryngeal specimen of patients with PCR technique. 33 % of patients in case group (24 patients) and 45.8 % (33 patients) of control group were positive for H. pylori. CagA gene was present in 13.8 % (10 patients) of case group specimens and 31.9 % (23 patients) of control group. This difference was statistically significant with Mantel–Haenszel statistical test analyses. The results showed that patients with LSCC have significantly lower incidence of laryngeal H. pylori infection and cagA virulence factor than those without LSCC. Findings from this study support the protective effect of H. pylori infection against laryngeal cancer.
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References
Dunn BE, Cohen H, Blaser MJ (1997) Helicobacter Pylori. Clin Microbiol Rev 10(4):720–741
Parsonnet J, Hansen S, Rodriguez L et al (1994) Helicobacter pylori infection and gastric lymphoma. N Engl J Med 330(18):1267–1271
Covacci A, Telford JL, DelGuidice G et al (1999) Helicobacter pylori virulence and genetic geography. Science 284:1328–1333
Kikuchi S, Crabtree JE, Forman D, Kurosawa M (1999) Association between infections with CagA-positive or -negative strains of Helicobacter pylori and risk for gastric cancer in young adults. research group on prevention of gastric carcinoma among young adults. Am J Gastroenterol 94(12):3455–3459
Blaser MJ, Perez-Perez GI, Kleanthous H et al (1995) Infection with Helicobacter pylori strains possessing cagA is associated with an increased risk of developing adenocarcinoma of the stomach. Cancer Res 55(10):2111–2115
Hatakeyama M, Higashi H (2005) Helicobacter pylori CagA: a new paradigm for bacterial carcinogenesis. Cancer Sci 96:835–843
Oleastro M, Gerhard M, Lopez Al et al (2003) H. pylori virulence genotypes in Portuguese children and adults with gastroduodenal pathology. Eur J Clin Microbiol Infect Dis 22(2):85–91
Cirak MY, Ozdek A, Yilmaz D et al (2003) Detection of Helicobacter pylori and its cagA gen in tonsils and adenoid tissue by PCR. Arch Otolaryngol Head Neck Surg 129(11):1225–1229
Wienholt MG, Erbling MC, Bennetts RW et al (1993) Detection of antibodies to Helicobacter pylori using oral fluid specimens. Ann N Y Acad Sci 694:340–342
Akbayir N, Basak T, Seven H et al (2005) Investigation of Helicobacter pylori colonization in laryngeal neoplasia. Eur Arch Otorhinolaryngol 262(3):170–172
Kosunen TU, Seppala K, Sarna S et al (1992) Diagnostic value of decreasing IgG, IgA and IgM antibody titers after eradication of Helicobacter pylori. Lancet 339(8798):893–895
Ozyrut M, Gunyor A, Ergunanay K, Cekin E, Erkul E et al (2009) Real time PCR detection of helicobacter pylori and virulence associated cagA in nasal polyps and laryngeal disorders. Otolaryngol Head Neck Surg 141(1):131–135
Aviv JE, Savita Collins (2010) Upper aerodigestive manifestations of gastroesophageal reflux disease. In: Flint PW et al (eds) Cummings otolaryngology-head & neck surgery, 5th edn. Mosby, Philadelpia, pp 894
Feldman M, Freidman LS, Sleisenger MH (2006) Sleisenger and fordtrans gastrointestinal and liver disease, 8th edn. Saunders, Philadelphia
Serag HB, Sonnenberg A (1998) Opposing time trends of peptic ulcer and reflux disease. Gut 43(3):327–333
Wu JC, Sung JJ, Chan FK et al (2000) Helicobacter pylori infection in association with milder gastroesophageal reflux disease. Aliment Pharmacol Ther 14(4):427–432
Majidi MR, Rezaei S, Hssanzadeh N et al (2007) The correlation between helicobacter pylori infection and squamous cell carcinoma of larynx and hypopharynx. Iran J Otorhinolaryngol 48(19):89–94
Nurgalieva ZZ, Graham DY, Dahlstrom KR et al (2005) A pilot study of helicobacter pylori infection and risk of laryngopharyngeal cancer. Head Neck 27(1):22–27
Aygenc E, Selcuk A, Celikkanat S et al (2001) The role of helicobacter pylori infection in the cause of squamous cell carcinoma of the larynx. Otolaryngol Head Neck Surg 125(5):520–521
Grandis RJ, Perez-Perez GI, Yu VL et al (1997) Lack of serologic evidence for Helicobacter pylori infection in head and neck cancer. Head Neck 19(3):216–218
Mokhtari N, Khajeh KM, Saadatnia H, Rajati M (2004) Studing the cause effect relation between helicobacter pylori and SCC of larynx and hypopharynx. Iran J otorhinolaryngol 35:27–32
Acknowledgement
This study was undertaken under the supervision of Kerman Medical University Research Centre and in cooperation with Kerman Department of Education. We are grateful to Ehsan Mehrabi Kermani for his assistance in English editing of the manuscript and Dr. Sarafi Nejad for his generous help in statistical analyses of the study.
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Amizadeh, M., Shamsadini, A., Arabzadeh, A. et al. Association of cagA Positive Helicobacter pylori Infection and Laryngeal Squamous Cell Carcinoma: A PCR Approach. Indian J Otolaryngol Head Neck Surg 67 (Suppl 1), 51–55 (2015). https://doi.org/10.1007/s12070-014-0750-2
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DOI: https://doi.org/10.1007/s12070-014-0750-2