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Compact, common path quantitative phase microscopic techniques for imaging cell dynamics

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Abstract

Microscopy using visible electromagnetic radiation can be used to investigate living cells in various environments. But bright field microscopy only provides two-dimensional (2D) intensity distribution at a single object plane. One of the ways to retrieve object height/thickness information is to employ quantitative phase microscopic (QPM) techniques. Interferometric QPM techniques are widely used for this. Digital holographic microscopy (DHM) is one of the state-of-the-art methods for quantitative three-dimensional (3D) imaging. Usually it is implemented in two-beam geometry, which is prone to mechanical vibrations. But to study dynamics of objects like red blood cells, one needs temporal stability much better than the fluctuations of the object, which the two-beam geometry fails to deliver. One way to overcome this hurdle is to use self-referencing techniques, in which a portion of the object beam will act as the reference beam. Here the development of self-referencing QPM techniques is described along with the results.

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Acknowledgements

A Anand acknowledges research funding through DST-FIST and UGC-DRS projects and also UGC major research grant (42-776/2013(SR)).

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Authors and Affiliations

  1. Applied Physics Department, Faculty of Technology and Engineering, M.S. University of Baroda, Vadodara, 390 001, India

    A ANAND, P VORA, S MAHAJAN, V TRIVEDI & V CHHANIWAL

  2. Center of Medical Physics and Biomedical Engineering, Medical University of Vienna, Waehringer Guertel 18-20, 4L, 1090, Vienna, Austria

    A SINGH & R LEITGEB

  3. Department of Electrical Engineering, University of Connecticut, Storrs, CT, 06269-4157, USA

    B JAVIDI

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  1. A ANAND
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  2. P VORA
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Correspondence to A ANAND.

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ANAND, A., VORA, P., MAHAJAN, S. et al. Compact, common path quantitative phase microscopic techniques for imaging cell dynamics. Pramana - J Phys 82, 71–78 (2014). https://doi.org/10.1007/s12043-013-0644-y

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  • DOI: https://doi.org/10.1007/s12043-013-0644-y

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