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Hydrophilic C terminus of Salicornia europaea vacuolar Na+/H+ antiporter is necessary for its function

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Abstract

Plant vacuolar Na+/H+ antiporters play important roles in cellular ion homeostasis, vacuolar pH regulation and sequestration of Na+ ions into the vacuole. Previous research showed that hydrophilic C-terminal region of Arabidopsis AtNHX1 negatively regulates the Na+/H+ transporting activity. In this study, we truncated the hydrophilic C terminus of a vacuolar Na+/H+ antiporter gene from Salicornia europaea (SeNHX1) to generate its derivative, SeNHX1- ΔC. Expression of SeNHX1 and SeNHX1- ΔC in yeast mutant showed that SeNHX1 significantly improved the tolerance to NaCl; however, the expression of SeNHX1- ΔC enormously decreased the tolerance to NaCl. Overall, these results suggest that the hydrophilic C-terminal region of SeNHX1 is required for Na+/H+ exchanging activity of SeNHX1.

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Acknowledgements

We thank Prof. Aimin Zhang, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, for kindly providing the Saccharomyces cerevisiae mutant GX1. This work was supported financially by the National Natural Science Foundation of China (nos 31271419 and 31271793) and the National Science and Technology Key Project of China on GMO Cultivation for New Varieties (no. 2014ZX08003-002B).

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Authors and Affiliations

  1. School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, People’s Republic of China

    GUANGXIA WU, XIAOWEI TIAN & QING ZHAO

  2. School of Environmental Science and Engineering, Tianjin University, Tianjin, 300072, People’s Republic of China

    GANG WANG, JING JI, HAILING GAO, JING LI & YURONG WANG

  3. Yaohua High School, Tianjin, 300040, People’s Republic of China

    YURONG WANG

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  1. GUANGXIA WU
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  2. GANG WANG
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  3. JING JI
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  4. XIAOWEI TIAN
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  5. HAILING GAO
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  6. QING ZHAO
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  7. JING LI
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  8. YURONG WANG
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Correspondence to GANG WANG or JING JI.

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[Wu G., Wang G., Ji J., Tian X., Gao H., Zhao Q., Li J. and Wang Y. 2014 Hydrophilic C terminus of Salicornia europaea vacuolar Na+/H+ antiporter is necessary for its function. J. Genet. 93, xx–xx]

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WU, G., WANG, G., JI, J. et al. Hydrophilic C terminus of Salicornia europaea vacuolar Na+/H+ antiporter is necessary for its function. J Genet 93, 425–430 (2014). https://doi.org/10.1007/s12041-014-0396-6

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  • DOI: https://doi.org/10.1007/s12041-014-0396-6

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