Abstract
The ad-8 gene of Neurospora crassa, in addition to being used for the study of purine biology, has been extensively studied as a model for gene structure, mutagenesis and intralocus recombination. Because of this there is an extensive collection of well-characterized N. crassa ad-8 mutants in the Fungal Genetics Stock Center collection. Among these are spontaneous mutants and mutants induced with X-ray, UV or chemical mutagens. The specific lesions in these mutants have been genetically mapped at high resolution. We have sequenced the ad-8 locus from 13 of these mutants and identified the molecular nature of the mutation in each strain. We compare the historical fine-structure map to the DNA and amino acid sequence of each allele. The placement of the individual lesions in the fine-structure map was more accurate at the 5′ end of the gene and no mutants were identified in the 3′ untranslated region of this gene. We additionally analysed ad-8 + alleles in 18 N. crassa strains subjected to whole-genome sequence analysis and describe the variability among Neurospora strains and among fungi and other organisms.
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Acknowledgements
The FGSC is supported by grant 0742713 from the US National Science Foundation. The authors wish to thank Dr D. Kasbekar for his pre-submission comments on the text.
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[Wiest A., McCarthy A. J., Schnittker R., and McCluskey K. 2012 Molecular analysis of mutants of the Neurospora adenylosuccinate synthetase locus. J. Genet. xx, xx–xx]
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WIEST, A., McCARTHY, A.J., SCHNITTKER, R. et al. Molecular analysis of mutants of the Neurospora adenylosuccinate synthetase locus. J Genet 91, 199–204 (2012). https://doi.org/10.1007/s12041-012-0175-1
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DOI: https://doi.org/10.1007/s12041-012-0175-1