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Diazinon degradation by a novel strain Ralstonia sp. DI-3 and X-ray crystal structure determination of the metabolite of diazinon

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Abstract

Diazinon is a widely used organophosphorus insecticide often detected in the environment. A highly effective diazinon-degrading Ralstonia sp. strain DI-3 was isolated from agricultural soil. Strain DI-3 can utilize dimethoate as its sole carbon source for growth and degrade an initial concentration of 100 mg·L−1 diazinon to non-detectable levels within 60 h in liquid culture. A small amount of second carbon source as co-substrate could slightly enhance the biodegradation of diazinon. In addition, a less toxic metabolic intermediate formed during the degradation of diazinon mediated by strain DI-3 was purified using thin-layer chromatography (TLC) and identified based on single-crystal X-ray diffraction analysis, allowing a degradation pathway for diazinon by pure culture to be proposed. Finally, this is the first providing authentic evidence to describe the metabolite.

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Acknowledgements

This work was supported by grants from the Chinese National Natural Science Foundation (31100083, 41501304), the Foundation for Young Talents in College of Anhui Province, the Natural Science Foundation from Educational Commission of Anhui Province (KJ2015A049), Provincial Natural Science Foundation of Anhui (1508085MC49), Scholar Backbone Supporting Plan of Huaibei Normal University, and Construction Project from College Scientific Research Innovation Team of Anhui Province—Ecological Restoration and Utilization of Coal Mining Subsidence Area.

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  1. College of Life Sciences, Huaibei Normal University, Huaibei, 235000, P.R of China

    Guangli Wang & Yuan Liu

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  1. Guangli Wang
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  2. Yuan Liu
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Correspondence to Yuan Liu.

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Corresponding editor: B Jagadeeshwar Rao

[Wang G and Liu Y 2016 Diazinon degradation by a novel strain Ralstonia sp. DI-3 and X-ray crystal structure determination of the metabolite of diazinon. J. Biosci.]

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Wang, G., Liu, Y. Diazinon degradation by a novel strain Ralstonia sp. DI-3 and X-ray crystal structure determination of the metabolite of diazinon. J Biosci 41, 359–366 (2016). https://doi.org/10.1007/s12038-016-9619-8

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  • DOI: https://doi.org/10.1007/s12038-016-9619-8

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