Journal of Biosciences

, Volume 39, Issue 3, pp 513–517

Phylogenetic diversity of Mesorhizobium in chickpea

  • Dong Hyun Kim
  • Mayank Kaashyap
  • Abhishek Rathore
  • Roma R Das
  • Swathi Parupalli
  • Hari D Upadhyaya
  • S Gopalakrishnan
  • Pooran M Gaur
  • Sarvjeet Singh
  • Jagmeet Kaur
  • Mohammad Yasin
  • Rajeev K Varshney
Article

DOI: 10.1007/s12038-014-9429-9

Cite this article as:
Kim, D.H., Kaashyap, M., Rathore, A. et al. J Biosci (2014) 39: 513. doi:10.1007/s12038-014-9429-9

Abstract

Crop domestication, in general, has reduced genetic diversity in cultivated gene pool of chickpea (Cicer arietinum) as compared with wild species (C. reticulatum, C. bijugum). To explore impact of domestication on symbiosis, 10 accessions of chickpeas, including 4 accessions of C. arietinum, and 3 accessions of each of C. reticulatum and C. bijugum species, were selected and DNAs were extracted from their nodules. To distinguish chickpea symbiont, preliminary sequences analysis was attempted with 9 genes (16S rRNA, atpD, dnaJ, glnA, gyrB, nifH, nifK, nodD and recA) of which 3 genes (gyrB, nifK and nodD) were selected based on sufficient sequence diversity for further phylogenetic analysis. Phylogenetic analysis and sequence diversity for 3 genes demonstrated that sequences from C. reticulatum were more diverse. Nodule occupancy by dominant symbiont also indicated that C. reticulatum (60%) could have more various symbionts than cultivated chickpea (80%). The study demonstrated that wild chickpeas (C. reticulatum) could be used for selecting more diverse symbionts in the field conditions and it implies that chickpea domestication affected symbiosis negatively in addition to reducing genetic diversity.

Keywords

Chickpea domestication nitrogen fixation rhizobia symbiosis 

Copyright information

© Indian Academy of Sciences 2014

Authors and Affiliations

  • Dong Hyun Kim
    • 1
  • Mayank Kaashyap
    • 1
  • Abhishek Rathore
    • 1
  • Roma R Das
    • 1
  • Swathi Parupalli
    • 1
  • Hari D Upadhyaya
    • 1
  • S Gopalakrishnan
    • 1
  • Pooran M Gaur
    • 1
  • Sarvjeet Singh
    • 2
  • Jagmeet Kaur
    • 2
  • Mohammad Yasin
    • 3
  • Rajeev K Varshney
    • 1
    • 4
  1. 1.International Crops Research Institute for the Semi-Arid Tropics (ICRISAT)PatancheruIndia
  2. 2.Punjab Agricultural UniversityLudhianaIndia
  3. 3.RAK College of AgricultureSehoreIndia
  4. 4.CGIAR Generation Challenge Program, C/o CIMMYTMexicoMexico