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Multiple proteins of White spot syndrome virus involved in recognition of β-integrin

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Abstract

The recognition and attachment of virus to its host cell surface is a critical step for viral infection. Recent research revealed that β-integrin was involved in White spot syndrome virus (WSSV) infection. In this study, the interaction of β-integrin with structure proteins of WSSV and motifs involved in WSSV infection was examined. The results showed that envelope proteins VP26, VP31, VP37, VP90 and nucleocapsid protein VP136 interacted with LvInt. RGD-, YGL- and LDV-related peptide functioned as motifs of WSSV proteins binding with β-integrin. The β-integrin ligand of RGDT had better blocking effect compared with that of YGL- and LDV-related peptides. In vivo assay indicated that RGD-, LDV- and YGL-related peptides could partially block WSSV infection. These data collectively indicate that multiple proteins were involved in recognition of β-integrin. Identification of proteins in WSSV that are associated with β-integrin will assist development of new agents for effective control of the white spot syndrome.

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Acknowledgements

This study was supported by Grant (No. 2012CB114401) of the National Basic Research Program (973) of China, Grant (No. 30871942) of the National Science Foundation of China, Grant (No. 201103034) of Shrimp Research in the Public Interest and Taishan Scholar Project Fund of Shandong.

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Authors and Affiliations

  1. Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, 266071, P. R. China

    Jing-Yan Zhang, Qing-Hui Liu & Jie Huang

  2. Shanghai Ocean University, 999 Hucheng Ring Road, Pudong, Shanghai, China

    Jing-Yan Zhang

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  1. Jing-Yan Zhang
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  2. Qing-Hui Liu
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  3. Jie Huang
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Corresponding author

Correspondence to Qing-Hui Liu.

Additional information

Corresponding editor: Shahid Jameel

MS received 22 July 2013; accepted 03 February 2014

Corresponding editor: Shahid Jameel

[Zhang J-Y, Liu Q-H and Huang J 2014 Multiple proteins of White spot syndrome virus involved in recognition of β-integrin. J. Biosci. 39 1–8] DOI 10.1007/s12038-014-9418-z

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Zhang, JY., Liu, QH. & Huang, J. Multiple proteins of White spot syndrome virus involved in recognition of β-integrin. J Biosci 39, 381–388 (2014). https://doi.org/10.1007/s12038-014-9418-z

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