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Molecular Cloning and Expression of a Cryptochrome Gene CiCRY-DASH1 from the Antarctic microalga Chlamydomonas sp. ICE-L

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Abstract

Cryptochromes (CRYs) are flavin-binding proteins that sense blue and near-ultraviolet light and participate in the photoreactions of organisms and the regulation of biological clocks. In this study, the complete open reading frame (ORF) of CiCRY-DASH1 (GenBank ID MK392361), encoding one kind of cryptochrome, was cloned from the Antarctic microalga Chlamydomonas sp. ICE-L. The quantitative real-time PCR study showed that the CiCRY-DASH1 had the highest expression at 5 °C and salinity of 32‰. The CiCRY-DASH1 was positively regulated by blue, yellow, or red light. Moreover, the CiCRY-DASH1 can positively respond to extreme polar day and night treatment and exhibit a certain circadian rhythm, which indicated that CiCRY-DASH1 participated in the circadian clock and its expression was regulated by circadian rhythms. And the CiCRY-DASH1 was more noticeably affected by ultraviolet-B radiation than ultraviolet-A radiation, indicating ultraviolet-B light does obvious damage to Antarctic microalgae.

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Acknowledgements

This work was supported by the National Key Research and Development Program of China (2018YFD0900705), the China Ocean Mineral Resources R&D Association (No. DY135-B2-14), the National Key Research and Development Program of China (2018YFD0901103), the Natural Science Foundation of China (41776203, 41576187), Basic Scientific Fund for National Public Research Institutes of China (2016Q10), Key Research and Development Program of Shandong Province (2018YYSP024, 2018GHY115039), Ningbo Public Service Platform for High-Value Utilization of Marine Biological Resources (NBHY-2017-P2), the Natural Science Foundation of Shandong (No. ZR2019BD023), and National Programme on Global Change and Air-sea Interaction (No. GASI-03-02-02-05).

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This research received no further external funding except that mentioned under Acknowledgments.

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  1. Xin Zhang and Zhou Zheng are listed as co-first authors and contributed equally to this work.

Authors and Affiliations

  1. School of Basic Medicine, Qingdao University, Qingdao, 266071, China

    Xin Zhang, Yingying He & Lina Liu

  2. First Institute of Oceanography, Ministry of Natural Resource, Qingdao, 266061, China

    Zhou Zheng, Changfeng Qu & Jinlai Miao

  3. Laboratory for Marine Drugs and Bioproducts of Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266237, China

    Zhou Zheng, Changfeng Qu & Jinlai Miao

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  1. Xin Zhang
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Correspondence to Jinlai Miao.

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Zhang, X., Zheng, Z., He, Y. et al. Molecular Cloning and Expression of a Cryptochrome Gene CiCRY-DASH1 from the Antarctic microalga Chlamydomonas sp. ICE-L. Mol Biotechnol 62, 91–103 (2020). https://doi.org/10.1007/s12033-019-00225-y

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