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SplitCore Technology Allows Efficient Production of Virus-Like Particles Presenting a Receptor-Contacting Epitope of Human IgE

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Abstract

Immunoglobulin E (IgE) plays a central role in type I hypersensitivity including allergy and asthma. Novel treatment strategy envisages development of a therapeutic vaccine designed to elicit autologous blocking antibodies against the IgE. We sought to develop an IgE-epitope antigen that induces antibodies against a receptor-contacting epitope on human IgE molecule. We designed the VLP immunogens which utilize hepatitis B virus core protein (HBcAg) as a carrier, and present arrays of the receptor-contacting epitopes of the human IgE on their surfaces. FG loop from the IgE domain Cε3 was engineered into the HBcAg. Two constructs explore a well-established approach of insertion into a main immunodominant region of the HBcAg. Third construct is different in that the carrier is produced in a form of an assembly of two polypeptide chains which upon expression remain associated in a stable VLP-forming subunit (SplitCore technology). No VLPs were isolated from E.coli expressing the IgE-epitope antigens with contiguous sequences. On the contrary, the SplitCore antigen carrying the FG loop efficiently formed the VLPs. Immunization of mice with the VLPs presenting receptor-contacting epitope of the IgE elicited antibodies recognizing the human IgE in ELISA.

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Acknowledgments

This work was supported by Grant #0112RK00352 “Study of immunogenicity of virus-like particles carrying epitopes of human immunoglobulin E” from the Ministry of Education and Science (MES) of the Republic of Kazakhstan.

Conflict of interest

The authors declare that there are no conflicts of interest.

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Authors and Affiliations

  1. National Center for Biotechnology, Valikhanova 13/1, 010000, Astana, Kazakhstan

    A. Zh. Baltabekova, Zh. S. Shagyrova, A. S. Kamzina, E. M. Ramanculov & A. V. Shustov

  2. Nazarbayev University Research and Innovation System (NURIS), AOE “Nazarbayev University”, 010000, Astana, Kazakhstan

    M. Voykov & Ye. Zhiyenbay

Authors
  1. A. Zh. Baltabekova
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  2. Zh. S. Shagyrova
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  3. A. S. Kamzina
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  4. M. Voykov
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  5. Ye. Zhiyenbay
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  6. E. M. Ramanculov
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  7. A. V. Shustov
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Correspondence to A. V. Shustov.

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Baltabekova, A.Z., Shagyrova, Z.S., Kamzina, A.S. et al. SplitCore Technology Allows Efficient Production of Virus-Like Particles Presenting a Receptor-Contacting Epitope of Human IgE. Mol Biotechnol 57, 746–755 (2015). https://doi.org/10.1007/s12033-015-9867-0

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