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Identification of a Xylitol Dehydrogenase Gene from Kluyveromyces marxianus NBRC1777

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Abstract

Xylitol dehydrogenase (XDH) (EC 1.1.1.9) is one of the key enzymes in the xylose fermentation pathway in yeast and fungi. A xylitol dehydrogenase gene (XYL2) encoding a XDH was cloned from Kluyveromyces marxianus NBRC 1777, and the in vivo function was validated by disruption and complementation analysis. The highest activity of KmXDH could be observed at pH 9.5 during 55°C. The values of k cat/K m indicate that KmXDH prefers NAD+ to NADP+ (k cat/K + m NAD 3681/min mM and k cat/K + m NADP 1361/min mM). The different coenzyme preference between KmXR and KmXDH caused an accumulation of NADH in the xylose utilization pathway. The redox imbalance may be one of the reasons to cause the poor xylose fermentation under oxygen-limited conditions in K. marxianus NBRC1777.

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Acknowledgments

This work was supported by a grant-in-aid from the National Natural Science Foundation of China (Grant No. 31070028), and the project was also sponsored by the National Basic Research Program of China (Grant No. 2011CBA00801), the Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry and Specialized Research Fund for the Doctoral Program of Higher Education of China (Grant No. 20093402120027).

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Authors and Affiliations

  1. School of Life Science, University of Science and Technology of China, Hefei, 230027, Anhui, People’s Republic of China

    Li Lulu, Wang Dongmei, Gao Xiaolian & Hong Jiong

  2. School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang, 621010, Sichuan, People’s Republic of China

    Zhang Ling

  3. Department of Biology and Biochemistry, University of Houston, Houston, TX, 77004-5001, USA

    Gao Xiaolian

  4. Hefei National Laboratory for Physical Science at the Microscale, Hefei, 230026, Anhui, People’s Republic of China

    Li Lulu, Gao Xiaolian & Hong Jiong

  5. Department of Biochemical Science and Technology, Faculty of Agriculture, Kagoshima University, 1-21-24 Korimoto, Kagoshima, 890-0065, Japan

    Tamaki Hisanori

  6. Research Institute of Natural Resources, Ishikawa Prefectural University, Nonoichi-cho, Ishikawa, 921-8836, Japan

    Kumagai Hidehiko

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  1. Li Lulu
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Correspondence to Hong Jiong.

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Lulu, L., Ling, Z., Dongmei, W. et al. Identification of a Xylitol Dehydrogenase Gene from Kluyveromyces marxianus NBRC1777. Mol Biotechnol 53, 159–169 (2013). https://doi.org/10.1007/s12033-012-9508-9

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