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Increased Expression of TRPC5 in Cortical Lesions of the Focal Cortical Dysplasia

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Abstract

Focal cortical dysplasias (FCDs) are frequently associated with the medical refractory epilepsy in both children and adults. Transient receptor potential canonical channel 5 (TRPC5), a receptor-operated cation channel, has been well recognized as a regulator in the central nervous system. Here, we examined the expression and cellular distribution of TRPC5 in the specimens from patients with FCDIa (n = 14), FCDIIa (n = 12), and FCDIIb (n = 12) compared with the age-matched control cortex (CTX). TRPC5 mRNA and protein levels were significantly higher in FCDs compared with CTX. Immunohistochemical data showed that TRPC5 was strongly expressed in the misshapen cells, particularly in neuronal microcolumns, dysmorphic neurons, and balloon cells. Moreover, the double-label immunofluorescence analyses demonstrated that TRPC5 localized on NeuN-positive neurons. In addition, its co-localization with glutamate and gamma-aminobutyric acid (GABA) indicated that TRPC5 was distributed on both glutamatergic and GABAergic neurons. Taken together, these results suggested that increased expression of TRPC5 in FCDs and the cell-specific distribution patterns of TRPC5 in the misshapen neurons in FCDs could potentially contribute to the epileptogenesis of FCDs.

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Acknowledgments

The authors sincerely thank the patients and their families for their participation in this work. This work was supported by National Natural Science Foundation of China (Nos. 81071043, 81100975, 81070953), Natural Science Foundation Project of CQ CSTC (No. 2011JJ0124), and China Postdoctoral Science Foundation (NO. 2012 T50850).

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Authors and Affiliations

  1. Department of Neuosurgry, Xinqiao Hospital, Third Military Medical University, 183 Xinqiao Main Street, Shapingba District, Chongqing, 400037, China

    Guang-Zhen Xu, Hai-feng Shu, Hai-Yun Yue, Da-Hai Zheng, Wei Guo & Hui Yang

  2. Department of Neurosurgery, Jinan Military General Hospital of PLA, 25 Shifan Road, Jinan, 250031, Shandong, China

    Guang-Zhen Xu

  3. Department of Neurosurgery, Chengdu Military General Hospital of PLA, Chengdu, 610083, Sichuan, China

    Hai-feng Shu

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  1. Guang-Zhen Xu
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  2. Hai-feng Shu
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  3. Hai-Yun Yue
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  4. Da-Hai Zheng
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  5. Wei Guo
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  6. Hui Yang
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Correspondence to Hui Yang.

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Guang-Zhen Xu and Hai-feng Shu contributed equally to this work.

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Xu, GZ., Shu, Hf., Yue, HY. et al. Increased Expression of TRPC5 in Cortical Lesions of the Focal Cortical Dysplasia. J Mol Neurosci 55, 561–569 (2015). https://doi.org/10.1007/s12031-014-0390-8

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  • DOI: https://doi.org/10.1007/s12031-014-0390-8

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