Abstract
Several articles have highlighted the potential involvement of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) in neurodegeneration by showing a non-glycolytic activity of GAPDH specifically in the brains of subjects with Alzheimer’s disease (AD). The novel aim of this study was to elucidate the critical role of GAPDH and its interaction with β-amyloid in the blood of Moroccan patients with familial AD (FAD) carrying presenilin mutations and in sporadic late onset AD (LOAD). Our results show a significant decrease in the activity of GAPDH in blood samples from patients with FAD as compared to sporadic cases and healthy controls. The expression level of GAPDH in brain specimens from mutant tau transgenic mice and patients with FAD was unchanged as compared to healthy controls. In contrast, the expression level of GAPDH in blood samples from mutant tau transgenic mice and patients with FAD was decreased as compared to sporadic cases and healthy controls. Moreover, there is an accumulation of β-amyloid aggregates in the blood samples of patients with FAD and an increase in amyloid fibrils in both the blood and brain samples of these patients. Our study adds new insight to previous ones by showing the involvement of GAPDH in AD, which may influence the pathogenesis of this neurodegenerative disease.
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Acknowledgments
We would like to thank Ranbanxy Morocco LCC for their partial financial support to Miss Nadia El Kadmiri. We thank Mr. Othman Rouissi at Ranbanxy Morocco LLC and the staff at the CHU IBN ROCHD Neurology Department in Casablanca for their technical assistance. We thank all the staff at CIBERNED—Investigations Center of Neurodegenerative Diseases—the Centro de Biologia Molecular “Severo Ochoa” (Madrid, Spain) for their technical assistance. We also thank the staff at the Medical Genetics Institute at the Universita Catholica in Rome, Italy, for their help in gene sequencing. We thank Dr. B. Elkhalfi at the LPGM for her help.
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The authors declare that no competing interests exist.
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The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. No additional external funding was received for this study.
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El Kadmiri, N., Cuardos, R., El Moutawakil, B. et al. A Proteomic Approach for the Involvement of the GAPDH in Alzheimer Disease in the Blood of Moroccan FAD Cases. J Mol Neurosci 54, 774–779 (2014). https://doi.org/10.1007/s12031-014-0374-8
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DOI: https://doi.org/10.1007/s12031-014-0374-8