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5-HT7 Receptors Are Involved in Neurogenic Dural Vasodilatation in an Experimental Model of Migraine

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Abstract

Neurogenic dural vasodilation has been demonstrated to play an important role in migraine. 5-HT7 receptors have been found on trigeminal nerve endings and middle meningeal arteries and demonstrated involved in the dilatation of meningeal arteries. The aim of the present study was to demonstrate whether 5-HT7 receptors are involved in neurogenic dural vasodilation in migraine. The neurogenic dural vasodilation model of migraine was used in this study. Unilateral electrical stimulation of dura mater was performed in anesthetized male Sprague–Dawley rats. Animals were pretreated with selective 5-HT7 receptor agonist AS19, 5-HT7 receptor antagonist SB269970, 5-HT1B/1D receptor agonist sumatriptan, or vehicles. Blood flow of the middle meningeal artery (MMA) was measured by a laser Doppler flowmetry. AS19 significantly increased the basal and stimulated blood flows of the middle meningeal artery following electrical stimulation of dura mater, and its effect was dose dependent at the early stage. SB269970 and sumatriptan significantly reduced the basal and stimulated blood flows of middle meningeal artery. The present study demonstrates for the first time that 5-HT7 receptors are involved in neurogenic dural vasodilation evoked by electrical stimulation of dura mater and maybe of relevance in the pathophysiology and treatment of migraine.

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Abbreviations

ANOVA:

Analysis of variance

AS19:

(2S)-(+)-5-(1, 3, 5- Trimethylpyrazol-4-yl)-2-(dimethylamino) tetralin

BI:

Baseline

CGRP:

Calcitonin gene-related peptide

DMSO:

Dimethyl sulfoxide

LSD:

Fisher’s least significance difference

NS:

Normal saline

MMA:

Middle meningeal artery

SB269970:

(2R)-1-[(3-Hydroxyphenyl) sulfonyl]-2-[2-(4-methyl-1-piperidinyl) ethyl] pyrrolidine hydrochloride

TRPV1:

Transient receptor potential vanilloid 1

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Acknowledgments

This study was supported by the National Natural Science Foundation of China (grant numbers 81100830, 81171101) and the Medical Science and Technology Project of Guangzhou (grant number 20121A011025).

Conflict of Interest

There is no conflict of interest to disclose.

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Authors and Affiliations

  1. Department of Neurology, Guangzhou First Municipal People’s Hospital, Guangzhou Medical University, No. 1 Panfu Road, Guangzhou, 510180, China

    Xiaojuan Wang, Rong Hu & Xiaoping Pan

  2. Department of Neurology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China

    Yannan Fang

  3. Department of Clinical Laboratory, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China

    Jianbo Liang & Miansheng Yan

Authors
  1. Xiaojuan Wang
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  2. Yannan Fang
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  3. Jianbo Liang
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  4. Miansheng Yan
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  5. Rong Hu
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  6. Xiaoping Pan
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Corresponding author

Correspondence to Xiaoping Pan.

Additional information

Xiaojuan Wang, Yannan Fang and Jianbo Liang contributed equally to this work.

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Wang, X., Fang, Y., Liang, J. et al. 5-HT7 Receptors Are Involved in Neurogenic Dural Vasodilatation in an Experimental Model of Migraine. J Mol Neurosci 54, 164–170 (2014). https://doi.org/10.1007/s12031-014-0268-9

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  • DOI: https://doi.org/10.1007/s12031-014-0268-9

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