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Weight loss reduces anti-ADAMTS13 autoantibodies and improves inflammatory and coagulative parameters in obese patients

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Abstract

Obese patients have been described at increased risk of thrombotic thrombocytopenic purpura, a disease caused by anti-ADAMTS13 autoantibodies. ADAMTS13 has a structure homology with the adipokine thrombospondin-1. We previously demonstrated an increased presence of anti-ADAMTS13 antibodies in obese patients. We aimed to study the changes induced by weight loss after bariatric surgery on some inflammatory and coagulative parameters and their link with anti-ADAMTS13 autoantibodies. We studied 100 obese patients before and after weight loss induced by bariatric surgery and 79 lean volunteers as controls. We measured anthropometric, metabolic and inflammatory parameters, thrombospondin-1, ADAMTS13 activity, anti-ADAMTS13 autoantibodies, Von Willebrand factor. At baseline, 13 % of patients was positive for anti-ADAMTS13 autoantibodies, while all controls were negative. Thrombospondin-1 levels were higher in obese subjects with than without antibodies, with a positive correlation between the two parameters. In multiple logistic regression analysis only thrombospondin-1 levels predicted positivity for anti-ADAMTS13 antibodies. After weight loss both anti-ADAMTS13 antibodies and thrombospondin-1 reduced significantly. Weight loss in obesity improves the inflammatory and coagulative profile, and in particular anti-ADAMTS13 autoantibodies, ADAMTS13 activity and thrombospondin-1.

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Acknowledgments

This work was supported by funding from MIUR PRIN—Programmi di Ricerca Scientifica di Rilevante Interesse Nazionale, anno 2010–2011—prot. 2010329EKE_005 given to Roberto Vettor.

Funding

This work was supported by funding from MIUR PRIN—Programmi di Ricerca Scientifica di Rilevante Interesse Nazionale, anno 2010–2011—prot. 2010329EKE_005 given to Roberto Vettor.

Contributions

AM L, RF and VZ wrote the manuscript. VZ also contributed to literature search and generation of figures. LB contributed to data analysis and interpretation. C DP, MF, LP, G BDM, contributed to data collection. FF and RV revised the manuscript. All authors had final approval of the submitted and published versions.

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Authors and Affiliations

  1. Department of Medicine—DIMED, University of Padova, Internal Medicine 3, Padova, Italy

    Veronica Zanato, Luca Busetto, Chiara Dal Prà, Roberto Vettor & Roberto Fabris

  2. Department of Medicine—DIMED, University of Padova, Internal Medicine 1, Padova, Italy

    Anna Maria Lombardi & Fabrizio Fabris

  3. Week Surgery, Hospital Establishment of Padova, Padova, Italy

    Mirto Foletto & Luca Prevedello

  4. Critical Area Department, Emergency Room Unit, Treviso, Montebelluna, Italy

    Giulia Berti De Marinis

Authors
  1. Veronica Zanato
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  2. Anna Maria Lombardi
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  3. Luca Busetto
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  4. Chiara Dal Prà
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  9. Roberto Vettor
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  10. Roberto Fabris
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Correspondence to Veronica Zanato.

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informed consent was obtained from all individual participants included in the study.

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All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

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Zanato, V., Lombardi, A.M., Busetto, L. et al. Weight loss reduces anti-ADAMTS13 autoantibodies and improves inflammatory and coagulative parameters in obese patients. Endocrine 56, 521–527 (2017). https://doi.org/10.1007/s12020-016-1059-6

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  • DOI: https://doi.org/10.1007/s12020-016-1059-6

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