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The Effects of Phrenic Nerve Degeneration by Axotomy and Crush on the Electrical Activities of Diaphragm Muscles of Rats

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Abstract

The aim of this study was to investigate the effect of axotomy and crush-related degeneration on the electrical activities of diaphragm muscle strips of experimental rats. In the present study, twenty-one male Wistar-albino rats were used and divided into three groups. The animals in the first group were not crushed or axotomized and served as controls. Phrenic nerves of the rats in the second and third groups were crushed or axotomized in the diaphragm muscle. Resting membrane potential (RMP) was decreased significantly in both crush and axotomy of diaphragm muscle strips of experimental rats (p < 0.05). Depolarization time (T DEP) and half-repolarization (1/2 RT) time were significantly prolonged in crush and axotomy rats (p < 0.05). Crushing or axotomizing the phrenic nerves may produce electrical activities in the diaphragm muscle of the rat by depolarization time and half-repolarization time prolonged in crush and axotomy rats.

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Acknowledgments

This work was supported by the Yüzüncü Yıl University Research Projects Department. We thank Dr. Nurettin Cengiz and Hava Bektaş.

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Authors and Affiliations

  1. Vocational College Technical Programmes, Muş Alparslan University, Muş, Turkey

    Mehmet Eşref Alkiş

  2. Department of Biophysics, Faculty of Medicine, Muğla Sıtkı Koçman University, Muğla, Turkey

    Servet Kavak

  3. Department of Thoracic Surgery, Faculty of Medicine, Yüzüncü Yıl University, Van, Turkey

    Fuat Sayır

  4. Department of Biophysics, Ondokuzmayis University, Medical School, Samsun, Turkey

    Aydin Him

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  1. Mehmet Eşref Alkiş
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  2. Servet Kavak
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Correspondence to Mehmet Eşref Alkiş.

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Alkiş, M.E., Kavak, S., Sayır, F. et al. The Effects of Phrenic Nerve Degeneration by Axotomy and Crush on the Electrical Activities of Diaphragm Muscles of Rats. Cell Biochem Biophys 74, 29–34 (2016). https://doi.org/10.1007/s12013-015-0708-3

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  • DOI: https://doi.org/10.1007/s12013-015-0708-3

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