Abstract
In this paper, we report the improvement of PCR microfluidic chip efficiency achieved by coating the inner surface of steel capillary microchannel with a 22-µm film of the ultraviolet-solidified NOA61 using a device invented by us. Our results indicate that with this treatment, the roughness of the inside wall of steel capillary was improved from Ra = 0.921 to Ra = 0.254. The contact angle was decreased from about 95° to 56°, and the surface hydrophobicity was also increased. The flow pressure for performing the real-time PCR in the microfluidic chip with modified surface was reduced by twofold (2.11/1) and that resulted in a substantially increased efficiency of PCR. A modification of the microchannel interior surface improved the quality of the on-chip integrated PCR procedure.
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Acknowledgments
This project was supported by the National Natural Science Foundation of China (Grant No. 51375024), Scientific-technical project of Beijing Education Committee (Grant No. KZ201210005009), and the opening foundation of the State Key Laboratory of Space Medicine Fundamentals and Application, Chinese Astronaut Research and Training Center (Grant No. SMFA13K06).
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Wu, J., Guo, W., Wang, C. et al. Research to Improve the Efficiency of Double Stereo PCR Microfluidic Chip by Passivating the Inner Surface of Steel Capillary with NOA61. Cell Biochem Biophys 72, 605–610 (2015). https://doi.org/10.1007/s12013-014-0508-1
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DOI: https://doi.org/10.1007/s12013-014-0508-1