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Simultaneous Detection of Four Common Oral Candida Species from Blood Samples by the Fluorescence Polarization Assay

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Abstract

The genus Candida is both the commensal microbe and the opportunistic pathogen, containing approximately 200 species inhabiting in oral cavity of 53 % of the general population. Candida species can cause the diseases from local mucosal infections to systemic mycoses, even life-threatening infections in immunocompromised individuals. The timely differentiation of Candida species is important for the guidance of clinical medication. Four common Candida species in Chinese population (Candida albicans, Candida tropicalis, Candida glabrata, Candida krusei) were chosen as the targets to develop the rapid screening method in this work. Combined with amplification by asymmetric PCR, this parallel fluorescence polarization (FP) immunoassay is carried out in homogeneous solution phase. The limit of detection of the assay was shown to be 50 copies/mL in blood samples. The evaluation in multicenter manner showed excellent reproducibility and stability. The comparison between DNA sequencing and the FP immunoassay indicated that there was no significant difference between these methods. This molecular strategy-based method is simple, rapid, and feasible for identifying common Candida species and thereby holding great potential in the application of clinical laboratories.

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Authors and Affiliations

  1. Department of Orthodontics, College of Stomatology, Shandong University, Jinan, 250012, China

    Yuhong He, Gaoyi Wu & Chunling Wang

  2. Department of Stomatology, Jinan Military General Hospital, Jinan, 250031, China

    Yuhong He, Peihuan Wang, Lanlan Xi, Zhaoling Wang & Gaoyi Wu

  3. Department of Dental Implantology Centre, School of Stomatology, Capital Medical University, Beijing, 100050, China

    Wei Geng

Authors
  1. Yuhong He
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  2. Wei Geng
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  3. Peihuan Wang
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  4. Lanlan Xi
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  5. Zhaoling Wang
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  6. Gaoyi Wu
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  7. Chunling Wang
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Correspondence to Gaoyi Wu or Chunling Wang.

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Yuhong He and Wei Geng have contributed equally to this paper and should be regarded as co-first author.

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He, Y., Geng, W., Wang, P. et al. Simultaneous Detection of Four Common Oral Candida Species from Blood Samples by the Fluorescence Polarization Assay. Cell Biochem Biophys 71, 919–924 (2015). https://doi.org/10.1007/s12013-014-0284-y

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  • DOI: https://doi.org/10.1007/s12013-014-0284-y

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