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Tegillarca granosa Extract Haishengsu Induces Apoptosis in Human Hepatocellular Carcinoma Cell Line BEL-7402 Via Fas-Signaling Pathways

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Abstract

This study was designed to investigate the apoptosis-inducing properties of Tegillarca granosa extract Haishengsu (HSS) in human hepatocellular carcinoma cell line BEL-7402. Proliferation inhibition of the human hepatocellular carcinoma BEL-7402 cells was determined by the MTT assay, and the cell viability was determined by trypan blue dye exclusion assay. Apoptosis of BEL-7402 cells was demonstrated by fluorescence microscope with flow cytometry with Annexin V-FITC/PI double staining and Hoechst 33258 staining. Western blot analysis and RT-PCR were used to determine the expression levels of Fas. Expressions of caspase-8 and caspase-3 were examined by caspase activity assay and western blot analysis. HSS inhibited the proliferation of human hepatocellular carcinoma BEL-7402 cells in a dose- and time-dependent manner. Our results showed HSS had positive effect on apoptosis through flow cytometry assay and fluorescence microscope. The expressions of Fas protein and mRNA were up-regulated following the treatment. Caspase-8 and caspase-3 were activated in the cells cultured with HSS. In conclusion, HSS induced apoptosis of human hepatocellular carcinoma BEL-7402 cells. The apoptosis was associated with the up-regulation of Fas and the activations of caspase-8 and caspase-3.

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Acknowledgments

This work was supported by the National Science Foundation of China Grant (Nos. 31100824 and 81473384); Shandong Provincial Natural Science Foundation (No. ZR2010HL068) and Qingdao Municipal Science and Technology Bureau (No. 12-1-3-39-nsh).

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None to declare.

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Authors and Affiliations

  1. Medical College of Qingdao University, Qingdao, 266071, China

    Xuehong Chen, Yantao Han & Chunbo Wang

  2. The Affiliated Hospital of Qingdao University, Qingdao, 266071, China

    Songmei Zhan

  3. Qingdao Haihui Biochemical Pharmaceutical Company, Qingdao, 266031, China

    Shouguo Chen

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  1. Xuehong Chen
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  2. Yantao Han
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  3. Songmei Zhan
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  4. Chunbo Wang
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  5. Shouguo Chen
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Correspondence to Xuehong Chen.

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Chen, X., Han, Y., Zhan, S. et al. Tegillarca granosa Extract Haishengsu Induces Apoptosis in Human Hepatocellular Carcinoma Cell Line BEL-7402 Via Fas-Signaling Pathways. Cell Biochem Biophys 71, 837–844 (2015). https://doi.org/10.1007/s12013-014-0271-3

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