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The Functions of Antioxidants and Heat Shock Proteins Are Altered in the Immune Organs of Selenium-Deficient Broiler Chickens

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Abstract

Despite increasing evidence indicating the essential involvement of selenium (Se) in the immune system, the effect of Se deficiency on the regulation of oxidative stress and heat shock proteins (Hsps) in broiler chickens is still unclear. In the present study, we established an exudative diathesis (ED) broiler chicken model caused by Se deficiency. We then analyzed histological observations and detected the expression levels of Hsps and antioxidant indexes in immune tissues. The antioxidant function declined remarkably, and most of the Hsp expression levels increased significantly in the spleen, thymus, and bursa of Fabricius of the broiler chicks with ED (except the messenger RNA (mRNA) levels of Hsp27, Hsp40, and Hsp70, which decreased in thymus tissues from the treatment groups); therefore, constitutive oxidation resistance and higher Hsps in broiler chicks with ED caused defects in immune organ morphology and function, as evidenced by abnormal histological structures: red pulp broadening and lymphocytes in the cortex and medulla of the thymic lobule decreased distinctly and distributed loosely. These results underscore the importance of Se in establishing an immune organ microenvironment conducive to normal function.

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Acknowledgments

The authors thank the members of the Veterinary Internal Medicine Laboratory, College of Veterinary Medicine, and Northeast Agriculture University for their help with sample collection.

Funding

This study was supported by the National Natural Science Foundation of China (Grant No. 31472161).

Conflict of Interest

The authors declare that they have no conflicts of interest.

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Authors and Affiliations

  1. College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, People’s Republic of China

    Zijiang Yang, Ci Liu, Weijia Zheng & Shu Li

  2. College of Animal Science and Technology, Northeast Agricultural University, Harbin, 150030, People’s Republic of China

    Xiaohua Teng

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  1. Zijiang Yang
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  2. Ci Liu
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  4. Xiaohua Teng
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Correspondence to Xiaohua Teng or Shu Li.

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Yang, Z., Liu, C., Zheng, W. et al. The Functions of Antioxidants and Heat Shock Proteins Are Altered in the Immune Organs of Selenium-Deficient Broiler Chickens. Biol Trace Elem Res 169, 341–351 (2016). https://doi.org/10.1007/s12011-015-0407-3

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