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Selenium Deficiency Influences the Gene Expressions of Heat Shock Proteins and Nitric Oxide Levels in Neutrophils of Broilers

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Abstract

The aim of the present study was to investigate the effects of selenium (Se) deficiency on the expressions of heat shock proteins (Hsp90, 70, 60, 40, and 27) and nitric oxide (NO) levels in neutrophils of broilers. One hundred eighty 1-day-old broilers were randomly assigned into two groups and were fed on a low-Se diet (0.008 mg/kg Se) or a control diet (0.2 mg/kg Se), respectively. Then, the messenger RNA (mRNA) levels of Hsp90, 70, 60, 40, and 27, induced nitric oxide synthase (iNOS), and NO levels were examined. The results showed that Se deficiency increased the mRNA levels of Hsps and iNOS and induced higher level of NO in chicken neutrophils (P < 0.05). It showed that the expression of Hsp40 increased higher than other Hsps in neutrophils, which indicated that it might play the crucial protective role in neutrophils. In addition, correlation analysis showed that iNOS had the biggest correlation with Hsp60, which indicated that Hsp60 might play an important function in inhibiting the production of NO, and the correlation coefficient between Hsp60 and Hsp70 was over 0.9, which indicated that they might have a synergistic effect. These results suggested that the level of NO and Hsp expression levels in neutrophils can be influenced by Se deficiency. And Hsp40 might play the crucial protective role in neutrophils induced by Se deficiency.

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Acknowledgments

This study was supported by the National Natural Science Foundation of China (31272626); the International (Regional) Cooperation and Exchange Projects of the National Natural Science Foundation of China (31320103920); the Study Abroad Foundation of Heilongjiang Province (LC201031); and the Doctoral Fund of the Ministry of Education of China (20122325110018).

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  1. College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, People’s Republic of China

    Xi Chen, Haidong Yao, Linlin Yao, Jinxin Zhao, Yilin Luan, Ziwei Zhang & Shiwen Xu

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  1. Xi Chen
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  2. Haidong Yao
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  4. Jinxin Zhao
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Correspondence to Ziwei Zhang or Shiwen Xu.

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Chen, X., Yao, H., Yao, L. et al. Selenium Deficiency Influences the Gene Expressions of Heat Shock Proteins and Nitric Oxide Levels in Neutrophils of Broilers. Biol Trace Elem Res 161, 334–340 (2014). https://doi.org/10.1007/s12011-014-0150-1

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