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Rapid Simultaneous Detection of Anti-protozoan Drugs Using a Lateral-Flow Immunoassay Format

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Abstract

This research describes the development of a multi-analyte lateral-flow immunoassay intended for the simultaneous detection of three anti-protozoan drugs (coccidiostats). These drugs, namely, halofuginone, toltrazuril and diclazuril, are used in the treatment of Eimeria spp. infections in cattle, pigs, chickens and turkeys. Coloured carboxylated microspheres were coated with each of the detection antibodies and employed in a lateral-flow assay format for detection of these residues in eggs. Using this approach, halofuginone was detectable at a limit of 10 ng/mL or greater, toltrazuril at 100 ng/mL and, similarly, diclazuril had a detection limit of 100 ng/mL, which is below the maximum allowed residue limit for all three as outlined by EU regulation. This simple cost-efficient assay and analysis method could pave the way for more efficient simultaneous monitoring of small-molecule residues in the future.

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Acknowledgments

This material is based on works supported by the Food Institutional Research Measure (FIRM) of the Department of Agriculture and Food (Ireland), Science Foundation Ireland (Grant 05/CE3/B754), and the Biomedical Diagnostics Institute (BDI), all of which are gratefully acknowledged.

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Authors and Affiliations

  1. School of Biotechnology and National Centre for Sensor Research, Dublin City University, Dublin 9, Ireland

    Jenny Fitzgerald, Paul Leonard & Richard O’Kennedy

  2. Biomedical Diagnostics Institute, Dublin City University, Dublin 9, Ireland

    Paul Leonard & Richard O’Kennedy

  3. Ashtown Food Research Centre, Ashtown, Dublin 15, Ireland

    Martin Danaher

Authors
  1. Jenny Fitzgerald
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  2. Paul Leonard
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  3. Martin Danaher
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  4. Richard O’Kennedy
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Correspondence to Richard O’Kennedy.

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Fitzgerald, J., Leonard, P., Danaher, M. et al. Rapid Simultaneous Detection of Anti-protozoan Drugs Using a Lateral-Flow Immunoassay Format. Appl Biochem Biotechnol 176, 387–398 (2015). https://doi.org/10.1007/s12010-015-1582-6

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  • DOI: https://doi.org/10.1007/s12010-015-1582-6

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