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Rhizopus oryzae Lipase Immobilized on Hierarchical Mesoporous Silica Supports for Transesterification of Rice Bran Oil

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Abstract

The tunable textural properties of self-oriented mesoporous silica were investigated for their suitability as enzyme immobilization matrices to support transesterification of rice bran oil. Different morphologies of mesoporous silica (rod-like, rice-like, and spherical) were synthesized and characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), and nitrogen adsorption–desorption isotherms. The surface area, pore size, and ordered arrangement of the pores were found to influence the immobilization and activity of the enzyme in the mesopores. The immobilization in rod-like silica was highest with an immobilization efficiency of 63 % and exhibited minimal activity loss after immobilization. Functionalization of the mesoporous surface with ethyl groups further enhanced the enzyme immobilization. The free enzyme lost most of its activity at 50 °C while the immobilized enzyme showed activity even up to 60 °C. Transesterified product yield of nearly 82 % was obtained for 24 h of reaction with enzyme immobilized on ethyl-functionalized SBA-15 at an oil:methanol ratio of 1:3. Fourier transform infrared spectroscopy (FT–IR) and Gas chromatography–mass spectrometry (GC-MS) were used to characterize the transesterified product obtained. The reusability of the immobilized enzyme was studied for 3 cycles.

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Acknowledgments

The authors wish to acknowledge the characterization facilities established from the PG Teaching fund of the Nanomission council, Department of Science and Technology, and infrastructure support from SASTRA University.

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Authors and Affiliations

  1. Centre for Nanotechnology and Advanced Biomaterials, School of Chemical and Biotechnology, SASTRA University, Thanjavur, 613401, India

    Prashanth Ramachandran, Guru Krupa Narayanan, Sakthivel Gandhi, Swaminathan Sethuraman & Uma Maheswari Krishnan

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  1. Prashanth Ramachandran
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  2. Guru Krupa Narayanan
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  3. Sakthivel Gandhi
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  4. Swaminathan Sethuraman
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  5. Uma Maheswari Krishnan
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Correspondence to Uma Maheswari Krishnan.

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Prashanth Ramachandran and Guru Krupa Narayanan have equal contribution to this study.

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Ramachandran, P., Narayanan, G.K., Gandhi, S. et al. Rhizopus oryzae Lipase Immobilized on Hierarchical Mesoporous Silica Supports for Transesterification of Rice Bran Oil. Appl Biochem Biotechnol 175, 2332–2346 (2015). https://doi.org/10.1007/s12010-014-1432-y

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  • DOI: https://doi.org/10.1007/s12010-014-1432-y

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