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The Aggregation of Aβ42 Induced by Nano Copper and the Antagonistic Action of Polysaccharides

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Abstract

The toxic effect of Aβ42 induced by copper nanoparticle (Cu NPs) was studied by atomic force microscopy (AFM), circular dichroism (CD) spectroscopy, and Thioflavin T (ThT) fluorescence technique. Five hundred nanometers of copper nanoparticle capped with polyvinylpyrrolidone (PVP) was used to evaluate the aggregation and fibrils of Aβ42. The morphologies of Aβ42 incubated in the presence of Cu NPs changed gradually. The aggregation and fibrils were observed in AFM images. However, in the presence of polysaccharides, the Cu NPs-induced fibrillation of Aβ42 was inhibited. Interestingly, the formed Cu NPs–polysaccharides complexes can even remodel the preformed Aβ42 fibrils into the low neurotoxic amorphous aggregates, which were maybe ascribed to the higher affinity of polysaccharides for Aβ42 than Cu NPs. Besides, it was found that the binding constant of Cu NPs to Aβ42 is smaller than that of polysaccharides. The relationship among polysaccharides, copper nanoparticle, and Aβ42 morphologies and its neurotoxicity were discussed, and the binding force was analyzed.

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Acknowledgments

We gratefully acknowledge the financial support of Scientific Research Foundation of Minnan Normal University (L20629) and Key Research Items of Fujian Province (2012N0031).

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Authors and Affiliations

  1. Department of Biological Sciences and BioTechnology, Minnan Normal University, Zhangzhou, 363000, China

    Wei Wang, Guoguang Zhang & Jinmei Zou

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  1. Wei Wang
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  2. Guoguang Zhang
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  3. Jinmei Zou
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Correspondence to Wei Wang.

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Wang, W., Zhang, G. & Zou, J. The Aggregation of Aβ42 Induced by Nano Copper and the Antagonistic Action of Polysaccharides. Appl Biochem Biotechnol 175, 1557–1566 (2015). https://doi.org/10.1007/s12010-014-1385-1

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  • DOI: https://doi.org/10.1007/s12010-014-1385-1

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